Aim: Effects of electromagnetic fields (Wi-Fi) on the pancreatic tissue of rats and the role of selenium and L-carnitine were investigated. Material and Method: Thirty male Wistar Albino rats were used in this study. The rats were divided randomly into 5 groups. Group I: EMR (n = 6): 28 days, 60 min / day was exposed to 2.45 GHz EMR. Group II: EMR + Selenium (n = 6): 28 days, 60 min / day was exposed to 2.45 GHz EMR and selenium 1.5 mg / kg / alternate day by intraperitoneal (i.p.) Group III EMR + L-carnitine (n = 6): 28 days, 60 min / day were exposed to 2.45 GHz EMR and L-carnitine 100 mg / kg / day by i.p. Group IV: Sham control (n = 6): 28 days 60 min / day in the experimental setup without EMR and saline 0.5 ml / day of i.p. was administered. Group V: Cage control (n = 6): 28 days without any application were kept under the same environmental conditions. At the end of the experiment the rats' blood samples were collected for amylase and lipase measurement and pancreas tissue was evaluated by histopathologically. Results: Amylase (p = 0.007) and lipase (p = 0.004) levels were found statistically significant between the groups. These parameters were increased related to EMR in Group I and amylase and lipase were significantly decreased in Group II and III. Histopathological evaluation; serous glands degeneration of the pancreas tissue (p = 0.009) and vascular congestion (p = 0.009) increased in the group I. These parameters were significantly decreased in group II and III. Discussions: EMR caused pancreatic tissue damage and selenium has been demonstrated protective effect more than L-carnitine.