This study was carried out to investigate the effects of different sulphur (S) and iron (Fe) sources on Fe nutrition of strawberry plants (Fragaria vesca L.). Therefore, elemental S and S containing waste from a S factory, corresponding to 500 and 1000 kg ha(-1) of S, and 20 kg ha(-1) of Fe, from Fe-SO, and Sequestrene Fe-138(Fe-chelate) were applied to the soil. Applications of elemental S and S containing waste resulted in a decrease of soil pH. While soil pH was 8.3 without S application, applied S corresponding to 500 and 1000 kg ha(-1) from both sources, decreased pH to 7.9 and 7.7 for elemental S and 7.9 and 7.8 for waste application, respectively. The leaf Fe concentration, chlorophyll content, green colour intensity and fruit yield increased with single and/ or combined applications of S and Fe. Both S sources showed similar effects regarding parameters mentioned above. The results showed that S containing waste could be used as an alternative to elemental S for improvement of Fe nutrition in calcareous soils under similar conditions.