Objective: Schizophrenia is a significant burden for the patient and causes great costs for society. The etiology of schizophrenia, which is known to be a neurodevelopmental disorder, has not been fully elucidated. Differences in prenatal gonadal hormones have been suggested to play a role in the pathogenesis of schizophrenia. An easy way to evaluate a biomarker that gives insight about prenatal androgen is the second to fourth digit ratio (2D:4D) of the hand. In this study, we aimed to compare the 2D:4D ratio of schizophrenia patients to healthy controls and to investigate the relationship with positive and negative symptoms.