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Non-Ionic Contrast Media Induces Oxidative Stress and Apoptosis Through Ca2+ Influx in Human Neutrophils

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dc.creator KAYAN, Mustafa
dc.creator Ovey, Ishak Suat
dc.creator YÜREKLİ, Vedat Ali
dc.creator Uguz, Abdulhadi Cihangir
dc.creator Aykur, Mehmet
dc.creator NAZIROĞLU, Mustafa
dc.date 2012-11-30T22:00:00Z
dc.date.accessioned 2020-10-06T11:00:17Z
dc.date.available 2020-10-06T11:00:17Z
dc.identifier ad2410fa-a092-4866-b6ec-c16c53ff1908
dc.identifier 10.1007/s00232-012-9491-x
dc.identifier https://avesis.sdu.edu.tr/publication/details/ad2410fa-a092-4866-b6ec-c16c53ff1908/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/69133
dc.description Non-ionic contrast media (CM) can induce tissue kidney injury via activation of phagocytosis and oxidative stress, although the mechanisms of injury via neutrophils are not clear. We investigated the effects of CM on oxidative stress and Ca2+ concentrations in serum and neutrophils of humans. Ten migraine patients were used in the study. Serum and neutrophil samples from patients' peripheral blood were obtained before (control) and 30 min after non-ionic (iopromide) CM injection. The neutrophils were incubated with non specific transient receptor potential 2 (TRPM2) channel blocker, 2-aminoethoxydiphenyl borate (2-APB), and voltage gated Ca2+ channel blockers, verapamil plus diltiazem. Serum and neutrophil lipid peroxidation, apoptosis and intracellular Ca2+ concentrations levels were higher in the CM group than in controls. The neutrophilic reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) levels as well as serum vitamin E and beta-carotene concentrations were lower in the CM group than in controls. Neutrophil lipid peroxidation levels were lower in the CM+2-APB and CM+verapamil-diltiazem groups than in the CM group, although GSH, GSH-Px and intracellular Ca2+ values increased in the CM+2-APB and CM+verapamil-diltiazem groups. However, caspase-3, caspase-9, vitamin A and vitamin C values were unaltered by CM treatment. In conclusion, we observed that CM induced oxidative stress and Ca2+ influx by decreasing vitamin E, beta-carotene and Ca2+ release levels in human serum and neutrophils. However, we observed protective effects of Ca2+ channel blockers on Ca2+ influx in neutrophils.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Non-Ionic Contrast Media Induces Oxidative Stress and Apoptosis Through Ca2+ Influx in Human Neutrophils
dc.type info:eu-repo/semantics/article

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