This research was conducted to determine the chemical composition of smooth bromegrass in artificial pasture from the years 2010 to 2012 in Isparta Province located in the Mediterranean region of Turkey. The mixture of the pasture used were alfalfa (Medicago sativa L.) + sainfoin (Onobrychis sativa Lam.) + crested wheatgrass (Agropyron cristatum L.) + smooth bromegrass (Bromus inermis L.). Animal grazing applications were performed in the second and the third year of the study since the first year covered only the establishment of the artificial pastures. Forage samples were collected from grazing and non-grazing areas once every 15 days during the grazing seasons. The crude protein (CP), acid detergent fibre (ADF), neutral detergent fibre (NDF) contents, total digestible nutrient (TDN) and relative feed value (RFV) were determined on the smooth bromegrass forage samples. According to results, CP, TDN and RFV values decreased throughout the grazing season, while ADF and NDF contents increased in grazing and non-grazing areas. The ADF and NDF contents of smooth bromegrass in non-grazed areas were higher than the grazed areas, while CP, TDN and RFV values of grazed areas were higher than non-grazed areas. It can be concluded that the harvesting at the late stages caused a reduction in forage quality of smooth bromegrass in grazing and non-grazing areas.