The objective of this study was to investigate the effects of replacing beef fat with enzymatic interesterified palm kernel oil (IPKO) on the quality characteristics and storage stability of sucuk (Turkish dry-fermented sausage). The partial (25, 50, 75%) and complete (100%) replacement of added beef fat with IPKO were carried out during sucuk processing. According to the study results, replacement of beef fat with IPKO resulted in significant increase in the concentrations of caprylic, capric, lauric and mryistic acid and decrease in the concentrations of stearic, oleic and linoleic acid in sucuk (P < 0.05). Even though replacement with IPKO did not affect TBARS of sucuk dough, a slightly higher TBARS values were determined in sucuk manufactured with IPKO at the end of storage compared to control (P < 0.05). Hardness, gumminess and chewiness values of sucuks were decreased with IPKO replacement (P < 0.05). Replacement with IPKO increased moisture and a* and b*, decreased protein, fat and pH in sucuks at the end of fermentation (P < 0.05).