| dc.creator |
Tukel, C |
|
| dc.creator |
Akcelik, M |
|
| dc.creator |
Tuncer, Y |
|
| dc.date |
2005-01-01T01:00:00Z |
|
| dc.date.accessioned |
2021-12-03T11:46:33Z |
|
| dc.date.available |
2021-12-03T11:46:33Z |
|
| dc.identifier |
a65b9b96-0c1f-4d45-bf26-64710508f44f |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/a65b9b96-0c1f-4d45-bf26-64710508f44f/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/93952 |
|
| dc.description |
High frequency conjugal transfer systems were analysed in lactococci using by 5 donor and two recipient strains with different phenotypic characters. High frequency transfer of plasmids was found to be associated with the formation of aggregation structures in donor-recipient strain mixtures in the conjugation experiments. Aggregation abilities of Lactococcus lactis strain mixtures were determined to be controlled with a two element-system one of which was encoded by lactose plasmids (Clu) and the other by chromosomal DNA (Agg). Conjugal transfer frequencies of plasmids were obtained at high levels such as 4.2 x 10(-2)-1.5 x 10(-4) per donor cell when aggregate forming donor-recipient mixtures were used. Plasmid stabilities were determined between 82-98 % in conjugants obtained from conjugation experiments in which L. lactis subsp. lactis MLL40-29 was used as recipient. A reduction in the production of a 48.9 kDa protein was determined with the disappearance of the clumping phenotype. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Analysis of the high frequency conjugal transfer systems in lactococci |
|
| dc.type |
info:eu-repo/semantics/article |
|