Objective The aim is to compare blood groups between spontaneously pregnant fertile patients and infertile female patients undergoing treatment for infertility diagnosis is defined as the failure to achieve pregnancy despite regular unprotected sexual intercourse for one year during the reproductive period without using any contraceptive method. Material and Method Our study includes statistically evaluating the blood groups and demographic data of a total of 608 patients, including 304 fertile patients who presented for routine pregnancy follow-up at the Department of Obstetrics and Gynecology, Süleyman Demirel University, between January 2015 and January 2023, and 304 infertile patients receiving treatment at the Süleyman Demirel University Assisted Reproductive Techniques (ART) Center. Results The mean age of the volunteers in the fertile group was calculated as 30.68±4.70, while the mean age of the volunteers in the infertile group was 32.50±5.35. Blood group A was found to be dominant in both the fertile and infertile groups, followed by blood groups O, B, and AB. When compared based on education, it was determined that the majority of patients in the infertile group had received undergraduate or postgraduate education. Income levels were categorized as low, medium, and high income based on occupational distributions in both groups, revealing that the majority of participants in both groups had a low-income level. Conclusion Infertility is a multifactorial condition resulting from a combination of various factors such as genetics, environment, lifestyle, and health. The prevalence of infertility increases with age, and blood group distribution does not have a direct impact on infertility.