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Protective efficacy of ramelteon on methotrexate-induced DNA damage

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dc.creator ASLAN KOŞAR, Pınar
dc.creator YAVUZ TÜREL, Gülçin
dc.date 2024-01-01T00:00:00Z
dc.date.accessioned 2025-02-25T10:32:47Z
dc.date.available 2025-02-25T10:32:47Z
dc.identifier 7dec1e09-6b0e-46f0-8682-9ffe53ded1a6
dc.identifier 10.1080/01480545.2024.2375300
dc.identifier https://avesis.sdu.edu.tr/publication/details/7dec1e09-6b0e-46f0-8682-9ffe53ded1a6/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/100290
dc.description Ramelteon (RMLT) is a melatonin receptor agonist that it has antioxidative and anti-inflammatory effects associated with DNA damage through different mechanisms of action. In this regard, we investigated the potential usefulness of RMLT as a protective agent against methotrexate (MTX)-induced DNA damage. Four groups were constituted from 32 Wistar albino rats: Negative control, RMLT, MTX, and MTX + RMLT. Twenty mg/kg MTX (i.p., single dose) and RMLT 10 mg/kg (oral, 7 days) was administered. Comet assay was used and the parameter %TailDNA was used to detect DNA damage. %TailDNA was 4.90 ± 0.19 in the control group, 7.85 ± 0.33 in the MTX group, 5.49 ± 0.24 in the RMLT group, and 5.86 ± 0.23 in the MTX + RMLT group. While there was a significant increase in DNA damage in the MTX-treated group compared to the control group, there was a significant reduction in DNA damage in the MTX + RMLT group, compared to the MTX group (p < 0.001). In conclusion, it was observed that combined treatment with RMLT significantly reduced MTX-induced DNA damage.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Protective efficacy of ramelteon on methotrexate-induced DNA damage
dc.type info:eu-repo/semantics/article


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