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Comparison of exosomes secreted by synovial fluid-derived mesenchymal stem cells and adipose tissue-derived mesenchymal stem cells in culture for microRNA-127-5p expression during chondrogenesis

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dc.creator ÇEMREK, FATİH
dc.creator Eker Sarıboyacı, Ayla
dc.creator Demir Güçlüer, Zilif
dc.creator SEVİMLİ, Murat
dc.creator İNAN, ULUKAN
dc.creator Nur Soykan, Merve
dc.creator Altuğ Tasa, Burcugül
dc.creator Qomi Ekenel, Emilia
dc.creator GÜNEŞ BAĞIŞ, SİBEL
dc.creator SEMERCİ SEVİMLİ, TUĞBA
dc.creator UYSAL, ONUR
dc.date 2023-05-20T00:00:00Z
dc.date.accessioned 2025-02-25T10:34:34Z
dc.date.available 2025-02-25T10:34:34Z
dc.identifier 9627159b-41ab-4b68-a602-b4fd00ba0813
dc.identifier 10.1016/j.gene.2023.147337
dc.identifier https://avesis.sdu.edu.tr/publication/details/9627159b-41ab-4b68-a602-b4fd00ba0813/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/100633
dc.description This study aimed to investigate the differences between the exosomal microRNA-127-5p expression profiles of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and human synovial fluid-derived mesenchymal stem cells (hSF-MSCs) during chondrogenesis in terms of regenerative treatment of cartilage. Synovial fluid-derived mesenchymal stem cells, adipose tissue-derived mesenchymal stem cells, and human fetal chondroblast cells (hfCCs) were directed to chondrogenic differentiation. Alcian Blue and Safranin O stainings were performed to detect chondrogenic differentiation histochemically. Exosomes derived from chondrogenic differentiated cells and their exosomes were isolated and characterized. microRNA-127-5p expressions were measured by Quantitative reverse transcription PCR (qRT-PCR). Significantly higher levels of microRNA-127-5p expression in differentiated hAT-MSCs exosomes, similar to human fetal chondroblast cells, which are the control group in the chondrogenic differentiation process, were observed. hAT-MSCs are better sources of microRNA-127-5p than hSF-MSCs for stimulating chondrogenesis or in the regenerative therapy of cartilage-related pathologies. hAT-MSCs exosomes are rich sources of microRNA-127-5p and can be an essential candidate for cartilage regeneration treatments.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Comparison of exosomes secreted by synovial fluid-derived mesenchymal stem cells and adipose tissue-derived mesenchymal stem cells in culture for microRNA-127-5p expression during chondrogenesis
dc.type info:eu-repo/semantics/article


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