| dc.creator |
Canseven, Vesile,
1989-
author
158793 |
|
| dc.creator |
Çubuk Demiralay, Ebru,
1976-
thesis advisor
14265 |
|
| dc.creator |
Süleyman Demirel Üniversitesi.
Fen Bilimleri Enstitüsü.
Kimya Anabilim Dalı.
9868
issuing body |
|
| dc.date |
2016. |
|
| dc.identifier |
http://tez.sdu.edu.tr/Tezler/TF03450.pdf |
|
| dc.description |
Present thesis was designed for determination of acid dissociation constants (pKa) of pravastatin, atorvastatin and rosuvastatin by using reversed phase liquid chromatography (RPLC) method. In this study, methanol-water binary mixture was chosen as hydroorganic solvent mixture. pH values were measured in the hydroorganic mixture, which was used as the mobile phase. The combined effect of the two factors (solvent percentage and pH of the mobile phase) on the chromatographic behavior of these compounds was investigated. The results obtained show that the dissociation constants increase with increasing organic modifier in mobile phase. Chromatographic study was performed with Nucleosil 300-5 C4 column (5μm, 250 mm 4.6 mm) and the flow rate of the experiment was adjusted at 1 mL/min. The acid dissociation constant values and the intrinsic retention factors were estimated by use of a non-linear regression model. The realibility of the RPLC methodology was investigated statistically using the difference between the experimental and theoretical results. Aqueous pKa values were also estimated with extrapolation by means of the Yasuda-Shedlovsky and mole fraction equations. The developed and validated method was successfully used for quantitative analysis of studied compounds. The high recovery and low relative standard deviation confirm the suitability of the method for determination of pravastatin, atorvastatin and rosuvastatin in tablet formulation. Keywords: Statins, Ionizaton constant, RPLC, Retention factor, Method validation |
|
| dc.description |
Present thesis was designed for determination of acid dissociation constants (pKa) of pravastatin, atorvastatin and rosuvastatin by using reversed phase liquid chromatography (RPLC) method. In this study, methanol-water binary mixture was chosen as hydroorganic solvent mixture. pH values were measured in the hydroorganic mixture, which was used as the mobile phase. The combined effect of the two factors (solvent percentage and pH of the mobile phase) on the chromatographic behavior of these compounds was investigated. The results obtained show that the dissociation constants increase with increasing organic modifier in mobile phase. Chromatographic study was performed with Nucleosil 300-5 C4 column (5μm, 250 mm 4.6 mm) and the flow rate of the experiment was adjusted at 1 mL/min. The acid dissociation constant values and the intrinsic retention factors were estimated by use of a non-linear regression model. The realibility of the RPLC methodology was investigated statistically using the difference between the experimental and theoretical results. Aqueous pKa values were also estimated with extrapolation by means of the Yasuda-Shedlovsky and mole fraction equations. The developed and validated method was successfully used for quantitative analysis of studied compounds. The high recovery and low relative standard deviation confirm the suitability of the method for determination of pravastatin, atorvastatin and rosuvastatin in tablet formulation. Keywords: Statins, Ionizaton constant, RPLC, Retention factor, Method validation |
|
| dc.description |
Tez (Yüksek Lisans) - Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, 2016. |
|
| dc.description |
Kaynakça var. |
|
| dc.description |
Present thesis was designed for determination of acid dissociation constants (pKa) of pravastatin, atorvastatin and rosuvastatin by using reversed phase liquid chromatography (RPLC) method. In this study, methanol-water binary mixture was chosen as hydroorganic solvent mixture. pH values were measured in the hydroorganic mixture, which was used as the mobile phase. The combined effect of the two factors (solvent percentage and pH of the mobile phase) on the chromatographic behavior of these compounds was investigated. The results obtained show that the dissociation constants increase with increasing organic modifier in mobile phase. Chromatographic study was performed with Nucleosil 300-5 C4 column (5μm, 250 mm 4.6 mm) and the flow rate of the experiment was adjusted at 1 mL/min. The acid dissociation constant values and the intrinsic retention factors were estimated by use of a non-linear regression model. The realibility of the RPLC methodology was investigated statistically using the difference between the experimental and theoretical results. Aqueous pKa values were also estimated with extrapolation by means of the Yasuda-Shedlovsky and mole fraction equations. The developed and validated method was successfully used for quantitative analysis of studied compounds. The high recovery and low relative standard deviation confirm the suitability of the method for determination of pravastatin, atorvastatin and rosuvastatin in tablet formulation. Keywords: Statins, Ionizaton constant, RPLC, Retention factor, Method validation |
|
| dc.description |
Present thesis was designed for determination of acid dissociation constants (pKa) of pravastatin, atorvastatin and rosuvastatin by using reversed phase liquid chromatography (RPLC) method. In this study, methanol-water binary mixture was chosen as hydroorganic solvent mixture. pH values were measured in the hydroorganic mixture, which was used as the mobile phase. The combined effect of the two factors (solvent percentage and pH of the mobile phase) on the chromatographic behavior of these compounds was investigated. The results obtained show that the dissociation constants increase with increasing organic modifier in mobile phase. Chromatographic study was performed with Nucleosil 300-5 C4 column (5μm, 250 mm 4.6 mm) and the flow rate of the experiment was adjusted at 1 mL/min. The acid dissociation constant values and the intrinsic retention factors were estimated by use of a non-linear regression model. The realibility of the RPLC methodology was investigated statistically using the difference between the experimental and theoretical results. Aqueous pKa values were also estimated with extrapolation by means of the Yasuda-Shedlovsky and mole fraction equations. The developed and validated method was successfully used for quantitative analysis of studied compounds. The high recovery and low relative standard deviation confirm the suitability of the method for determination of pravastatin, atorvastatin and rosuvastatin in tablet formulation. Keywords: Statins, Ionizaton constant, RPLC, Retention factor, Method validation |
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| dc.language |
tur |
|
| dc.publisher |
Isparta : Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü, |
|
| dc.subject |
Süleyman Demirel Üniversitesi |
|
| dc.title |
Dislipidemi tedavisinde kullanılan bazı ilaçların RPLC yöntemiyle iyonlaşma sabitlerinin tayini = Determination of ionization constants of some drugs used to treatment of dyslipidemia with RPLC method / |
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| dc.type |
text |
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