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Expression Profile of Transcription Factor ELK-1 and ELK-1 Target Genes on Lymphoma-Leukemia Cell Lines

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dc.creator YASAR SIRIN, Duygu
dc.creator AKALAN, Hande
dc.date 2018-08-15T00:00:00Z
dc.date.accessioned 2019-07-09T11:59:01Z
dc.date.available 2019-07-09T11:59:01Z
dc.identifier http://dergipark.org.tr/sdufenbed/issue/38975/456311
dc.identifier 10.19113/sdufbed.99971
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/46079
dc.description Prognostic molecular markers identified in leukemia are becoming increasingly important especially in risk stratification and to determine therapy. In this study, we investigate the role of ELK-1 transcription factor and its potential target genes in four cell lines; Daudi, Jurkat, K-562 and HL-60. To evaluate ELK-1, MCPIP, MCL-1, BCL-10, CEBPB and SRF genes expression profiles we have performed a Real-time PCR analysis on Daudi, Jurkat, K-562 and HL-60 cell lines. ELK-1 over expression concomitant with SRF overexpression was detected only in Daudi cell line while only SRF overexpression was detected in jurkat cells. Expression of MCPIP, MCL-1, BCL-10 and CEBPB genes were decreased in all cell lines. Protein levels or phosphorylation status of ELK-1, BCL-10, CEBPB, MCL-1, MCPIP and SRF, moreover, changes that may occur when ELK-1 continuous overexpression is provided or completely silenced in these cell lines have not been evaluated. These questions are suggestions for future investigations.
dc.format application/pdf
dc.publisher Süleyman Demirel University
dc.publisher Süleyman Demirel Üniversitesi
dc.relation http://dergipark.org.tr/download/article-file/528902
dc.source Volume: 22, Issue: 2 388-392 en-US
dc.source 1308-6529
dc.subject ELK-1,ELK-1 Target Genes; Expression profile
dc.title Expression Profile of Transcription Factor ELK-1 and ELK-1 Target Genes on Lymphoma-Leukemia Cell Lines en-US
dc.type info:eu-repo/semantics/article


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