| dc.creator |
SEVİM, Elif |
|
| dc.creator |
SEVİM, Ali |
|
| dc.date |
2015-09-02T10:18:12Z |
|
| dc.date.accessioned |
2019-07-09T12:00:34Z |
|
| dc.date.available |
2019-07-09T12:00:34Z |
|
| dc.identifier |
http://dergipark.org.tr/sdufenbed/issue/20807/222302 |
|
| dc.identifier |
10.19113/sdufbed.22055 |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/46804 |
|
| dc.description |
Fifteen Bacillus strains which were isolated from soil samples were examined for resistance to 17 different antibiotics (ampicillin, methicillin, erythromycin, norfloxacin, cephalotine, gentamycin, ciprofloxacin, streptomycin, tobramycin, chloramphenicol, trimethoprim-sulfamethoxazole, tetracycline, vancomycin, oxacilin, neomycin, kanamycin and, novabiocin) and to 10 different heavy metals (copper, lead, cobalt, chrome, iron, mercury, zinc, nickel, manganese and, cadmium) and for the presence of plasmid DNA. A total of eleven strains (67%) were resistant to at least one antibiotic. The most common resistance was observed against methicillin and oxacillin. The most resistance strains were found as Bacillus sp. B3 and Bacillus sp. B11. High heavy metal resistance against copper, chromium, zinc, iron and nickel was detected, but mercury and cobalt resistance was not detected, except for 3 strains (B3, B11, and B12) which showed mercury resistance. It has been determined that seven Bacillus strains have plasmids. The isolated plasmids were transformed into the Bacillus subtilis W168 and it was shown that heavy metal and antibiotic resistance determinants were carried on these plasmids. These results showed that there was a correlation between plasmid content and resistance for both antibiotic and heavy metal resistance |
|
| dc.description |
Fifteen Bacillus strains were isolated from soil samples in Rize, Turkey and, they were identified using morphological, biochemical and, molecular techniques. All strains were examined for resistance to 17 different antibiotics (ampicillin, methicillin, erythromycin, norfloxacin, cephalotine, gentamycin, ciprofloxacin, streptomycin, tobramycin, chloramphenicol, trimethoprim-sulfamethoxazole, tetracycline, vancomycin, oxacilin, neomycin, kanamycin, novabiocin) and to 10 different heavy metals (copper, lead, cobalt, chrome, iron, mercury, zinc, nickel, manganese, cadmium) and for the presence of plasmid DNA. A total of eleven strains (67%) were resistant to at least one antibiotic. The most common resistance was observed against methicillin and oxacillin. The most resistance strains were found as Bacillus sp. B3 and Bacillus sp. B11. High heavy metal resistance against copper, chromium, zinc, iron and nickel was detected, but mercury and cobalt resistance was not detected, except for 3 strains (B3, B11, B12) which showed mercury resistance. It has been determined that seven Bacillus strains have plasmids. The isolated plasmids were transformed into the Bacillus subtilis W168 and it was shown that heavy metal and antibiotic resistance determinants were carried on these plasmids. There was correlation between plasmid content and resistance for both antibiotic and heavy metal resistance. |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
Süleyman Demirel University |
|
| dc.publisher |
Süleyman Demirel Üniversitesi |
|
| dc.relation |
http://dergipark.org.tr/download/article-file/194072 |
|
| dc.source |
Volume: 19, Issue: 2
133-141 |
en-US |
| dc.source |
1308-6529 |
|
| dc.subject |
Bacillus Plasmid Antibiotic resistance Metal resistance Transformation |
|
| dc.subject |
Bacillus Plazmit Antibiyotik direnci Metal direnci Transformasyon |
|
| dc.title |
Plasmid Mediated Antibiotic and Heavy Metal Resistance in Bacillus Strains Isolated From Soils in Rize, Turkey |
en-US |
| dc.type |
info:eu-repo/semantics/article |
|