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Inheritance and detoxification enzyme levels in Tetranychus urticae Koch (Acari: Tetranychidae) strain selected with chlorpyrifos

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dc.creator Ay, RECEP
dc.creator Yorulmaz, SİBEL
dc.date 2010-04-30T21:00:00Z
dc.date.accessioned 2020-10-06T09:24:39Z
dc.date.available 2020-10-06T09:24:39Z
dc.identifier 0d18f1a9-1c2e-4f87-a638-93cac16ffc66
dc.identifier 10.1007/s10340-009-0274-9
dc.identifier https://avesis.sdu.edu.tr/publication/details/0d18f1a9-1c2e-4f87-a638-93cac16ffc66/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/53163
dc.description Changes in insecticide susceptibilities and detoxifying enzyme activities were measured in a strain of Tetranychus urticae Koch following repeated exposure to the organophosphate insecticide, chlorpyrifos. Twelve consecutive selection at the LC60 of the parental strain increased resistance from 8.58 to 91.45 fold. The interaction of some synergists [piperonyl butoxide, triphenyl phosphate and S-benzyl-O,O-diisopropyl phosphorothioate (IBP)] with chlorpyrifos was analyzed in the selected strain. Solely IBP showed a low synergistic effect with chlorpyrifos. The selected strain also demonstrated resistance against abamectin, propargite, clofentezine and fenpyroximate. The mode of resistance inheritance to chlorpyrifos was found to be incompletely dominant, and not sex-linked. Non-specific esterase enzyme activity was raised from 19.35 to 33.59 mOD/min/mg proteins during the selection period and it was observed that esterase band intensities visualized by polyacrylamide gel electrophoresis increased. This study has investigated the selection of resistance to chlorpyrifos and documented resistance to abamectin, propargite, clofentezine and fenpyroximate in Turkish T. urticae. Esterase enzymes may be playing a role in chlorpyrifos resistance while glutathione S-transferase (GST) and P450 enzymes do not appear to have any significant involvement.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Inheritance and detoxification enzyme levels in Tetranychus urticae Koch (Acari: Tetranychidae) strain selected with chlorpyrifos
dc.type info:eu-repo/semantics/article


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