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The phenolic compounds of petals and leaf tissue samples were determined in apple cvs. 'Breaburn', 'Golden Reinders', 'Granny Smith' and 'Jonagold' grafted on M9; 'Summerred' and 'William's Pride' grafted on M.26. Petals of apple flowers were taken at the pink bloom floral stage in April. Moreover leaves were sampled from the middle part of the annual shoots in July. The phenolic compounds were analyzed by High Pressure Liquid Chromatography (HPLC) technique. The gallic acid, p-hydroxy benzoic acid, eriodictyol, quercetin, ferulic acid, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid and apigenin-7-glucoside contents were determined in petal samples. The gallic acid, p-hydroxy benzoic acid, eriodictyol, quercetin, ferulic acid and p-coumaric acid were also investigated in leaf tissues. The concentrations of the compounds were influenced by the genotypes as well as by tissue samples. While eriodictyol was the predominant phenolic compound in leaves ranging between 156.75-414.90 mu g/g DW, chlorogenic acid was the predominant phenolic compound found only in petals ranging between 7784.60-19293.00 mu g/g DW in all cultivars investigated in this research. It was determined that the petals of apples were quite richer than the leaf samples in terms of the phenolic contents. Among the studied cultivars the total concentrations of phenols were higher in both the petal and leaf tissues of 'Granny Smith' apple cultivar. |
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