| dc.creator |
Charyyev, Muge Gok |
|
| dc.creator |
Tuncer, Banu Ozden |
|
| dc.creator |
Kankaya, Didem Akpinar |
|
| dc.creator |
TUNCER, Yasin |
|
| dc.date |
2019-02-28T21:00:00Z |
|
| dc.date.accessioned |
2020-10-06T09:27:29Z |
|
| dc.date.available |
2020-10-06T09:27:29Z |
|
| dc.identifier |
1941079e-cb88-44a6-956c-833689ff61ca |
|
| dc.identifier |
10.1007/s00003-019-01213-9 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/1941079e-cb88-44a6-956c-833689ff61ca/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/54388 |
|
| dc.description |
<p>The objectives of this study were to characterize the antibacterial substances produced by Enterococcus faecium YT52<br />isolated from boza, and to evaluate the safety of this strain. E. faecium YT52 inhibits various Gram-positive bacteria,<br />including foodborne pathogens such as Listeria monocytogenes and Bacillus cereus. The manner of action of its<br />antibacterial activity, using proteolytic enzymes, indicate that it produces a bacteriocin. The bacteriocin producing isolate<br />E. faecium YT52 was identified using 16S rRNA gene homology and species-specific polymerase chain reaction (PCR)<br />analysis. The bacteriocin was found to be heat stable and active under both acid and alkaline conditions. The bacteriocin<br />showed primary metabolite kinetics and a bactericidal mode of action against L. monocytogenes. Although enterocin A, B<br />and X genes were detected in E. faecium YT52 by PCR, only one active peptide band (* 5.5 kDa) with a molecular<br />weight similar to enterocin B was identified by tricine–SDS-PAGE analysis. A safety evaluation of E. faecium YT52<br />indicated that it is nonhemolytic, gelatinase-negative, and susceptible to clinically relevant antibiotics such as ampicillin,<br />tetracycline and vancomycin. It contains a small number of virulence factors (efaAfm, silent gelE) and antibiotic resistance<br />genes (ermB, tetM, tetL). In conclusion, bacteriocinogenic E. faecium YT52 was found to have a low risk profile, and it<br />may be potentially valuable as a protective culture in the food industry.</p> |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Bacteriocinogenic properties and safety evaluation of Enterococcus faecium YT52 isolated from boza, a traditional cereal based fermented beverage |
|
| dc.type |
info:eu-repo/semantics/article |
|