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The RNA-dependent RNA polymerase of Citrus tristeza virus forms oligomers

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dc.creator Cevik, Bayram
dc.date 2013-11-30T22:00:00Z
dc.date.accessioned 2020-10-06T09:29:33Z
dc.date.available 2020-10-06T09:29:33Z
dc.identifier 1c59fbb4-80e3-4424-aa42-efb433348fa8
dc.identifier 10.1016/j.virol.2013.08.029
dc.identifier https://avesis.sdu.edu.tr/publication/details/1c59fbb4-80e3-4424-aa42-efb433348fa8/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/54710
dc.description The RNA-dependent RNA polymerases (RdRp) from Citrus tristeza virus (CTV) were tagged with HA and FLAG epitopes. Differentially tagged proteins were expressed either individually or concomitantly in Escherichia coli. Immunoprecipitation of the expressed proteins with anti-FLAG antibody followed by Western blot with anti-HA antibody demonstrated that molecules of RdRp from CTV interact to form oligomers. Yeast two-hybrid assays showed that molecules of RdRp interact in eukaryotic cells. Co-immunoprecipitation with anti-FLAG antibody of truncated HA-tagged RdRps (RdRp Delta 1-166-HA, RdRp Delta 1-390-HA, RdRp1-169-HA) co-expressed with full-length RdRp-FLAG showed that only RdRp1-169-HA interacted with the full-length FLAG-RdRp. Yeast two-hybrid assays with truncated RdRp constructs confirmed that the oligomerization site resides in the N-terminal region and that the first 169 aa of CTV RdRp are necessary and sufficient for oligomerization both in bacterial and yeast cells. Development of control strategies targeting viral RdRp oligomer formation may inhibit virus replication and prove useful in control of CTV. (C) 2013 Elsevier Inc. All rights reserved.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title The RNA-dependent RNA polymerase of Citrus tristeza virus forms oligomers
dc.type info:eu-repo/semantics/article

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