Bio-dissolution, a potential tool of the hydrometallurgical industry, is an emerging biotechnology to add value to the mineral material processing sector. The microorganisms generally employed are iron and/or sulphur oxidizing acidophiles that are hardly contaminated with unwanted microbes. This is what makes them industrially important thereby finding novel applications in several sectors. In addition, adaptation to heavy metals is a unique biotechnological feature of these microbes. Keeping in view of the current scenario that is aimed at producing zero waste eco-friendly technologies, the present study aims at evaluating a biotechnological approach (as an alternative to the conventional process) for metal recovery from pyritic ask It is interesting to note that pyritic ash is an industrial waste (a byproduct) formed as a result of roasting of the pyrite ores that is associated with several environmental issues. The material contains some valuable metals which can be recovered. Arsenic has been chosen as the target metal of interest, in the present work, for noticing the dissolution patterns from the waste material using microbial approach. A mixed culture of acidophilic bacteria was used in the current work to evaluate the bio-dissolution efficacy of arsenic from the waste material. Optimum conditions were established using shaking flask reactors and it was found that about 60% of arsenic could be recovered from the sample using bio-dissolution technique.