| dc.creator |
Tola, Esra Nur |
|
| dc.creator |
MUNGAN, Muhittin Tamer |
|
| dc.creator |
UĞUZ, Abdulhadi Cihangir |
|
| dc.creator |
NAZIROĞLU, Mustafa |
|
| dc.date |
2012-12-31T22:00:00Z |
|
| dc.date.accessioned |
2020-10-06T09:35:59Z |
|
| dc.date.available |
2020-10-06T09:35:59Z |
|
| dc.identifier |
299016a2-901a-4e80-9663-672efe33cef7 |
|
| dc.identifier |
10.1071/rd12144 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/299016a2-901a-4e80-9663-672efe33cef7/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/56036 |
|
| dc.description |
Oxidative stress is important for promoting oocyte maturation and ovulation within the follicle through calcium ion (Ca2+) influx. The relationship between antioxidant and cytosolic Ca2+ levels and oocyte quality and fertilisation rate in the granulosa cells of patients undergoing in vitro fertilisation was investigated. Granulosa cells were collected from 33 patients. Cytosolic free Ca2+ ([Ca2+](i)) concentration, lipid peroxidation, reduced glutathione, glutathione peroxidase and oocyte quality were measured in the granulosa cells. The relationship between two drug protocols was also examined (gonadotrophin-releasing hormone antagonist and agonist protocols) and the same parameters investigated. The [Ca2+](i) concentration (P < 0.001), glutathione (P < 0.05) and oocyte quality (P < 0.001) values were significantly higher in the fertilised group than in the non-fertilised group, although glutathione peroxidase activity was significantly (P < 0.05) higher in the non-fertilised group than in the fertilised group. The [Ca2+](i) concentrations were also higher (P < 0.001) in the good-quality oocyte groups than in the poor-quality oocyte group. There was no correlation between the two drug protocols and investigated parameters. In conclusion, it was observed that high glutathione and cytosolic Ca2+ concentrations in granulosa cells of patients undergoing in vitro fertilisation tended to increase the fertilisation potential of oocytes. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Intracellular Ca2+ and antioxidant values induced positive effect on fertilisation ratio and oocyte quality of granulosa cells in patients undergoing in vitro fertilisation |
|
| dc.type |
info:eu-repo/semantics/article |
|