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Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA External Quality Assessment National Program Results

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dc.creator Karatayli, Ersin
dc.creator Soydemir, Ege
dc.creator Aksoy, Zeynep Busra
dc.creator Kizilpinar, Mehtap
dc.creator Altay Kocak, Aylin
dc.creator Karatayli, Senem Ceren
dc.creator Yurdcu, Esra
dc.creator Yildirim, Umut
dc.creator Guriz, Haluk
dc.creator BOZDAYI, GÜLENDAM
dc.creator Yurdaydin, Cihan
dc.creator İLHAN, OSMAN
dc.creator Yildirim, Yasin
dc.creator BOZDAYI, ABDURRAHMAN MİTHAT
dc.creator Oguz, Acelya Yalcintas
dc.creator Baris, Ahmet
dc.creator ALP, ALPASLAN
dc.creator Aksozek, Alper
dc.creator Sayiner, Arzu
dc.creator Karagul, Aydan
dc.creator Ordu, Aylin
dc.creator Istanbullu, Aye
dc.creator Otlu, Baris
dc.creator ARIDOĞAN, Buket
dc.creator Aksu, Burak
dc.creator BURUK, CELAL KURTULUŞ
dc.creator Karahan, Ceren
dc.creator Guney, Cakir
dc.creator Toksoz, Devrim
dc.creator Yildirim, Dilara
dc.creator ÇOLAK, DİLEK
dc.creator Daglar, Duygu Eren
dc.creator FINDIK, DUYGU
dc.creator Kas, Elif
dc.creator YILDIZ ZEYREK, FADİLE
dc.creator ÇALIŞKAN, EMEL
dc.creator Arslan, Fatma
dc.creator Demir, Feyza
dc.creator KİBAR, FİLİZ
dc.creator Milletli, Fikriye
dc.creator Ozdincer, Furkan
dc.creator Dundar, Gulnur
dc.creator Arslan, Hande
dc.creator Agca, Harun
dc.creator Güdücüoğlu, Hüseyin
dc.creator Aliskan, Hikmet Eda
dc.creator FİDAN, IŞIL
dc.creator Akyar, Isin
dc.creator Afsar, Ilhan
dc.creator KALELİ, İlknur
dc.creator Donmez, Ismail
dc.creator Midilli, Kenan
dc.creator Yanik, Kemalettin
dc.creator Cubukcu, Kivanc
dc.creator Ozdemir, Mehmet
dc.creator Acar, Melek
dc.creator Yalinay, Meltem
dc.creator Kuskucu, Mert Ahmet
dc.creator BAKICI, MUSTAFA ZAHİR
dc.creator AYDIN, Neriman
dc.creator YILMAZ, NEZİHA
dc.creator Ceken, Nihan
dc.creator Ziyade, Nihan
dc.creator Yilmaz, Nisel
dc.creator Ozgumus, Osman Birol
dc.creator Demirgan, Recep
dc.creator Gitmisoglu, Ozlem
dc.creator Kesli, Recep
dc.creator Guckan, Ridvan
dc.creator Sertoz, Ruchan
dc.creator AKGÜN, SADIK
dc.creator Aksaray, Sebahat
dc.creator TEZCAN ÜLGER, SEDA
dc.creator KAYGUSUZ, SEDAT
dc.creator GÖKAHMETOĞLU, SELMA
dc.creator Mese, Sevim
dc.creator Bayik, Seyit Ahmet
dc.creator AKÇALI, SİNEM
dc.creator GÜRCAN, ŞABAN
dc.creator KARSLIGİL, TEKİN
dc.creator Us, Tercan
dc.creator ÖZEKİNCİ, TUNCER
dc.creator Pilgir, Tulin
dc.creator Aslan, Ugur
dc.creator Dinc, Ugur
dc.creator Coskun, Umut Safiye Say
dc.creator Cetinkol, Yeliz
dc.creator Keskin, Yusuf
dc.creator Ayaydin, Zeynep
dc.creator AŞÇI TORAMAN, ZÜLAL
dc.date 2018-09-30T21:00:00Z
dc.date.accessioned 2020-10-06T10:24:34Z
dc.date.available 2020-10-06T10:24:34Z
dc.identifier 5ec350ba-196f-405e-b51b-81d056ed676e
dc.identifier 10.5578/mb.67081
dc.identifier https://avesis.sdu.edu.tr/publication/details/5ec350ba-196f-405e-b51b-81d056ed676e/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/61371
dc.description MOTAKK, as a national external quality control program has been launched to evaluate the molecular detection of viral infections including HBV DNA and HCV RNA in molecular microbiology diagnostic laboratories in Turkey. This program is prepared in compliance with ISO 17043:2010 (Conformity assessment general requirements for proficiency testing) standards, and aims to take the place of external quality control programs from abroad, contributing to standardization and accuracy of molecular diagnostic tests in our country. The aim of this study was to evaluate 2015 and 2016 results of the MOTAKK External Quality Control Program for HBV DNA and HCV RNA viral load. The calls were announced on the web page of MOTAKK (www.motakk.org). The quality control samples were sent to participating laboratories in 2015 and 2016. Main stocks were prepared from patients with chronic hepatitis B and C who had viral load detection with reference methods according to WHO reference materials for viral load studies to improve quality control sera. From these main stocks, samples with different viral loads were prepared from dilutions of plasma with HBV, HCV, HAV, HIV, Parvovirus B19 and CMV negative serologic markers. Quality control samples were sent to the participating laboratories along with the negative samples in the cold chain. The laboratories accomplished the related tests within 2-3 weeks and entered their results on the MOTAKK web page. These results were analysed according to ISO 13528 (Statistical methods for use in proficiency testing by interlaboratory comparison) and scoring reports were created by a software developed by MOTAKK and sent to participating labs. Each laboratory evaluated their own results in comparison with the other laboratory results, reassessed the tests via observing the distance from the mean result and the reference values. The number of laboratories participating in the HBV DNA and HCV RNA external quality control program was 70-73 in 2015-2016. Participants were able to comply with the program tools, registering, entering results and receiving the results reports problem. In HBV panel, 72.6-89.1% and 84.7-90.3% of the participant laboratories were in 1 standard deviation (SD) in 2015-2016, respectively. In HCV panel, 70.8-89.1% and 84.7-90.3% of the participant laboratories were in 1 SD in 2015-2016, respectively. A national external quality control program for HBV DNA and HCV RNA in Turkey has been prepared for the first time with this project and implemented successfully. All the data provided in the MOTAKK external quality control program final report, compensate all the data provided by the quality control program final reports from abroad; additionally, the report allows comparison of used technologies and commercial products.
dc.language tur
dc.rights info:eu-repo/semantics/openAccess
dc.title Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA External Quality Assessment National Program Results
dc.type info:eu-repo/semantics/article


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