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Isolation and expression analysis of three different flowering genes (TtLFY, TtAP1, and TtAP2) from an unusual legume species, Thermopsis turcica

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dc.creator KARGIOĞLU, MUSTAFA
dc.creator Kahraman, Busra
dc.creator Karakurt, Yasar
dc.creator CENKCİ, SÜLEYMAN
dc.creator Dedeoglu, Alperen
dc.date 2015-12-31T22:00:00Z
dc.date.accessioned 2020-10-06T10:51:18Z
dc.date.available 2020-10-06T10:51:18Z
dc.identifier a527ca0f-4089-49cb-a9bb-c4371ff367eb
dc.identifier 10.3906/bot-1509-15
dc.identifier https://avesis.sdu.edu.tr/publication/details/a527ca0f-4089-49cb-a9bb-c4371ff367eb/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/68334
dc.description LEAFY (LFY), APETALA1 (AP1), and APETALA2 (AP2) genes encode three different transcription factors that control and regulate flower initiation and development in Arabidopsis. By using 3'- and 5'-RACE analysis, we isolated and sequentially characterized TtLFY (a LEAFY-like gene), TtAP1 (a MADS-box-like gene), and TtAP2 (an AP2/ERBF-like gene) in Thermopsis turcica, an unusual endemic legume species with three free carpellated flower structure. Semiquantitative RT-PCR analysis for 18 different vegetative and reproductive tissues of T. turcica indicated TtLFY transcripts mainly in the shoot tips and young floral buds and TtAP1 transcripts in the sepals and petals; however, TtAP2 transcripts were detected in all tissues. This is the first record for a LFY-like gene, TtLFY, expressed in the shoot tips of an underground plant section and for an AP2-like gene transcript found in all tissues, similar to a housekeeping gene.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Isolation and expression analysis of three different flowering genes (TtLFY, TtAP1, and TtAP2) from an unusual legume species, Thermopsis turcica
dc.type info:eu-repo/semantics/article


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