| dc.creator |
BAYRAM, Dilek |
|
| dc.creator |
CALAPOĞLU, Mustafa |
|
| dc.creator |
ÖZGÖÇMEN, Meltem |
|
| dc.creator |
ARMAĞAN, İlkay |
|
| dc.creator |
ŞENOL, Nurgül |
|
| dc.date |
2017-12-31T21:00:00Z |
|
| dc.date.accessioned |
2020-10-06T11:00:41Z |
|
| dc.date.available |
2020-10-06T11:00:41Z |
|
| dc.identifier |
b0239555-c423-4f5b-bdd7-f13fde4b7f41 |
|
| dc.identifier |
10.1615/jenvironpatholtoxicoloncol.2018025226 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/b0239555-c423-4f5b-bdd7-f13fde4b7f41/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/69435 |
|
| dc.description |
This study aimed to investigate the effects of juglone on the human bladder carcinoma cell lines TCC-SUB and RT-4 in monolayer and spheroid cultures. Cells were treated with juglone at 24,48, and 72 h of incubation. The activity of caspase-3 was detected in vitro using a caspase-3 colorimetric assay kit according to the manufacturer's instructions. The bromodeoxyuridine (BrdU) labeling index was used to determine the cells of the synthesis phase. The terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay was used to determine the death of cells in both the monolayer and spheroid cultures. The control group had a large S-phase fraction and many of the TCC-SUB and RT-4 cells nuclei were observed to be positive for BrdU. The dead cell count was higher in the TCC-SUB and RT-4 cell lines with juglone applied than in the controls. We conclude that juglone significantly inhibits the proliferation and induces the apoptosis of TCC-SUB and RT-4 cells in vitro. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Determination of Apoptotic Effect of Juglone on Human Bladder Cancer TCC-SUP and RT-4 Cells: An In Vitro Study |
|
| dc.type |
info:eu-repo/semantics/article |
|