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Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization

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dc.creator Sezer, Serdar
dc.creator Koklukaya, Selva Zeynep
dc.creator Aksoy, Serpil
dc.creator HASIRCI, NESRİN
dc.date 2016-08-31T21:00:00Z
dc.date.accessioned 2020-10-06T11:01:30Z
dc.date.available 2020-10-06T11:01:30Z
dc.identifier b616f307-4ab4-4c65-b436-fb05b35de5dc
dc.identifier 10.1002/bab.1417
dc.identifier https://avesis.sdu.edu.tr/publication/details/b616f307-4ab4-4c65-b436-fb05b35de5dc/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/70045
dc.description In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carrageenan (P(AAm-CA)/K-car) semi-interpenetrating network by entrapment method. Optimum pH and temperatures values were determined between 5.0-6.0 and 40-50 degrees C for free laccase (FL) and immobilized laccases, respectively. After 42 days of storage at 4 degrees C, FL and immobilized laccases retained their original activities in the range of 55%-73%. Percent decolorization of Acid Orange 52 by free enyzme and enyzmes immobilized in hydrogels was found between 63% and 39%. Percent decolorization of Acid Orange 52 in the presence of mediator by free enyzme was found 73% and enyzmes immobilized in hydrogels were found as 73%. (C) 2015 International Union of Biochemistry and Molecular Biology, Inc.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization
dc.type info:eu-repo/semantics/article


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