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In vitro and in vivo antifungal activity of Satureja cuneifolia ten essential oil on Saprolegnia parasitica strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) eggs

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dc.creator Gormez, Oznur
dc.creator Terzioglu, Sedef
dc.creator Diler, Oznur
dc.creator Metin, Secil
dc.creator Didinen, Behire Isil
dc.date 2015-05-31T21:00:00Z
dc.date.accessioned 2020-10-06T11:37:49Z
dc.date.available 2020-10-06T11:37:49Z
dc.identifier e54350be-d3cf-42bf-ba12-a556210cb85b
dc.identifier 10.1111/are.12293
dc.identifier https://avesis.sdu.edu.tr/publication/details/e54350be-d3cf-42bf-ba12-a556210cb85b/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/74674
dc.description In the present study, the chemical composition and the antifungal properties against Saprolegnia parasitica (in vitro and in vivo) of the essential oils of thyme (Satureja cuneifolia) from Mediterranean region of Turkey were evaluated for the first time. The composition of oils was analysed using gas chromatography/mass spectrometry (GC/MS). The major constituents of oil of S.cuneifolia were cavracrol (46,84%) and cymene (16.90%). Antifungal effects of S.cuneifolia essential oil against S.parasitica strains (A1 and E1) were detected by disc diffusion and tube dilution assays. The antifungal effect of S.cuneifolia was determined to be stronger against S.parasitica E1 isolate (MIC 50LmL(-1), MLC 250LmL(-1)) compared with S.parasitica A1 isolate (MIC 50LmL(-1), MLC 500LmL(-1)). Following in vitro assays, effective doses of S.cuneifolia for disease control in rainbow trout eggs experimentally infected with S.parasitica were investigated. For this aim, infected eggs were treated with the essential oil (0, 5, 10, 20 and 50ppm) during incubation period (21days) after fertilization. Formalin (5mLL(-1)) was used as positive control. Hatching rate of eggs at the end of incubation period were calculated. The highest hatching rates were recorded in S.parasitica E1 strain at 5 and 10ppm concentrations of S.cuneifolia and in S.parasitica A1 strain at 10 and 20ppm (P<0.05).
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title In vitro and in vivo antifungal activity of Satureja cuneifolia ten essential oil on Saprolegnia parasitica strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) eggs
dc.type info:eu-repo/semantics/article


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