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An In Vitro Germination Technique for Some Stone Fruit Species: the Embryo Isolated from Cotyledons Successfully Germinated without Cold Pre-treatment of Seeds

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dc.creator San, BEKİR
dc.creator Yildirim, Adnan Nurhan
dc.creator Yildirim, FATMA
dc.date 2014-02-28T22:00:00Z
dc.date.accessioned 2020-10-06T11:38:14Z
dc.date.available 2020-10-06T11:38:14Z
dc.identifier e8528917-fba7-4773-b7f7-3c8b368640df
dc.identifier 10.21273/hortsci.49.3.294
dc.identifier https://avesis.sdu.edu.tr/publication/details/e8528917-fba7-4773-b7f7-3c8b368640df/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/74982
dc.description Seeds of temperate fruit species need a long time to germinate as a result of their requirement of stratification or cold treatment. Therefore, fast and uniform germination techniques are desirable and important for fruit tree propagation and especially for breeding studies. The effects of combinations of benzylaminopurine (BAP) and gibberellic acid (GA(3)) on in vitro embryo germination of apricot, peach, and wild cherry were determined without seed cold pretreatment. The results showed that no germination was recorded in all the seeds with testa. In the seeds without testa, no germination (wild cherry) or limited germination (less than 10%) was recorded. When the embryos separated from cotyledons were cultured, successful germination was obtained for all species. In general, the addition of different combinations of BAP and GA(3) into the Murashige and Skoog (MS) medium significantly increased the germination ratios of embryos without cotyledons in all species. For wild cherry, the best treatment (66.7% germination) was MS media containing 0.5 mg.L-1 BAP + 2.0 mg.L-1 GA(3) or 0.5 mg.L-1 BAP + 4.0 mg.L-1 GA(3). For peach, the best treatment (86.7% germination) was MS medium supplemented with 0.5 mg.L-1 BAP + 3.0 mg.L-1 GA(3). For apricot, the best treatment (93.3%) was MS media containing 0.5 mg.L-1 BAP + 3.0 mg.L-1 GA(3), 1.0 mg.L-1 BAP + 1.0 mg.L-1 GA(3), or 1.0 mg.L-1 BAP + 2.0 mg.L-1 GA(3).
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title An In Vitro Germination Technique for Some Stone Fruit Species: the Embryo Isolated from Cotyledons Successfully Germinated without Cold Pre-treatment of Seeds
dc.type info:eu-repo/semantics/article


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