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<p><span style="color: rgb(51, 51, 51); font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 12.6px;">Objective: The discovery of quorum sensing systems regulating bacterial virulence has afforded a novel opportunity to control infections without interfering with growth. As many bacteria Pseudomonas aeruginosa also used this system synthesized of some virulence factor such as alkaline protease, pyocyanin, phospholipase and exotoxin A, biofilm formation and swarming motility. Swarming motility is one of the major virulence factors; it is known to play a role in early biofilm development. Material-Method: In this study, inhibitory effect of methanol (MeOH), methanol-chloroform (MeOH-CHCl3) and water (H2O) extracts of the Rheum ribes L. on swarming motility in P. aeruginosa PA01 was investigated. And also antibacterial activity and minimum inhibitory concentration (MIC) values were investigated on Gram-positive Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 25923, Metisilin-Resistant Staphylococcus aureus ATCC 43300 and Gram-negative Chromobacterium violaceum ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PA01. Results: As a result, all extracts showed antibacterial effect on Gram-positive bacteria in different rate and according to swarming motility experiments, the highest inhibitory effect was seen in methanol and water extracts of Rheum ribes with 81%. Also chloroform extract showed similar inhibition rate (78%) on swarming motility. Conclusion: It is thought that the root extract of the plant has antibacterial properties especially on Gram positive bacteria, inhibits the swarming motility in P. aeruginosa PA01 and may have the potential to be a model for new generation drugs in the fight against infectious diseases.</span><br></p> |
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