| dc.creator |
Basaran, Pervin |
|
| dc.creator |
Ozcan, Meltem |
|
| dc.date |
2008-01-01T01:00:00Z |
|
| dc.date.accessioned |
2021-12-03T11:15:31Z |
|
| dc.date.available |
2021-12-03T11:15:31Z |
|
| dc.identifier |
15caf4f9-fdbf-47c4-8b1e-42b6d7a04577 |
|
| dc.identifier |
10.1016/j.biortech.2006.11.056 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/15caf4f9-fdbf-47c4-8b1e-42b6d7a04577/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/90103 |
|
| dc.description |
p-Xylosidase production was maximal for the mutant Pichia stipitis NP54376 grown on xylan as the sole carbon source. P-Xylosidase was purified from culture supernatant by (NH4)(2)SO4 precipitation and a hydrophobic interaction chromatography on phenyl sepharose. Optima of pH and temperature were 5.0 and 50 degrees C, respectively. The enzyme was inhibited by 2-mercaptoethanol (100%) and Fe3+ (80%), and moderately affected by Cu2+, Ag+, NH4+ and Mg2+ and SDS. The purified xylosidase hydrolyzed xylobiose and xylo-oligosaccharides and it did not exhibit activity against cellulose, starch, maltose and cellobiose. 2.5 g l(-1) glucose repressed P-xylosidase activity in the NP54376 strain. The K-m and V-max values on p-nitrophenyl-p-xylopyranoside were 1.6 mM and 186 mu mol p-nitrophenyl min(-1) mg(-1) protein, respectively. Analysis of the hydrolysis products by HPLC indicated that the major hydrolysis product is xylobiose in all the carbon sources tested. (C) 2006 Elsevier Ltd. All rights reserved. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Characterization of beta-xylosidase enzyme from a Pichia stipitis mutant |
|
| dc.type |
info:eu-repo/semantics/article |
|