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An improved Aceto-Iron-Haematoxylin staining for mitotic chromosomes in Cornelian cherry (Cornus mas L.)

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dc.creator Guloglu, Derya
dc.creator Zarifi, Eissa
dc.date 2016-01-01T01:00:00Z
dc.date.accessioned 2021-12-03T11:32:04Z
dc.date.available 2021-12-03T11:32:04Z
dc.identifier 8f4b4806-855c-4a98-a95d-fb4f629b5f4c
dc.identifier 10.1080/00087114.2015.1109930
dc.identifier https://avesis.sdu.edu.tr/publication/details/8f4b4806-855c-4a98-a95d-fb4f629b5f4c/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/93292
dc.description Karyotype analysis is considered an important prerequisite for fruit tree genetic and breeding studies. In this study observations were carried out on Cornus mas L. genotypes from Arasbaran forests and Kaleibar district in Iran. The root tips were pretreated with 8-Hydroxiquinolin, then fixed in Lewitsky solution and stained in Aceto-Iron-Haematoxylin (composed of 4% haematoxylin and 1% iron alum in 45% acetic acid). Ten metaphasic plates were used to analyze karyotype parameters. The results confirmed the existence of 2n = 2x = 18 chromosomes. The karyotypic formula was 2n = 2x = 18 = 8m + 2sm(sat) + 8sm, with the centromeres having median and sub median position. Chromosome size ranged between 11.33 +/- 0.54 to 6.60 +/- 0.19 mu m. The length of haploid complement was 71.54 mu m. Centromeric index were varied from 24.27 to 46.53, while the arm ratio values comprised in 1.15-2.72 intervals.
dc.language eng
dc.rights info:eu-repo/semantics/openAccess
dc.title An improved Aceto-Iron-Haematoxylin staining for mitotic chromosomes in Cornelian cherry (Cornus mas L.)
dc.type info:eu-repo/semantics/article


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