DSpace Repository

VURGULU ELEKTRİK ALAN (PEF) TEKNİĞİNİN ÇİĞ SÜTTE MİKROBİYAL LİPAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİNLİĞİNİN ARAŞTIRILMASI

Show simple item record

dc.creator AKAR, Nurullah Zekeriya; İstanbul Gelişim Üniversitesi
dc.date 2021-09-21T00:00:00Z
dc.date.accessioned 2021-12-03T11:45:44Z
dc.date.available 2021-12-03T11:45:44Z
dc.identifier https://dergipark.org.tr/tr/pub/jesd/issue/64976/876275
dc.identifier 10.21923/jesd.876275
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/93733
dc.description Süt ihtiva ettiği yüksek oranda su, nötr pH ve besleyici bileşenler ile mikroorganizmalar ve enzimler için ideal bir ortamdır. Çiğ sütü mikroorganizmalardan arındırmak ve istenmeyen kusurlara neden olan enzimleri inaktif etmek ve ayrıca sütün raf ömrünü uzatmada en bilinen muhafaza yöntemi ısıl işlemdir. Süt içerisindeki doğal enzimler pastörizasyon sıcaklığında inaktif olmakta ancak bakteriyel kaynaklı enzimler ise bu sıcaklığa dayanıklıdır. Yüksek derecedeki ısıl işlem normları sütün hem besin öğelerinde kayıplara hem de tekstür bozukluklarına neden olmaktadır. Aynı zamanda uzun süreli depolama ile ısıl işleme dirençli psikrofilik ve mezofilik bakteri kaynaklı enzimlerin oluşumu meydana gelmektedir. Bu enzimlerin başında süt teknolojisinde çeşitli tekstürel ve teknolojik sorunlara sebep olan lipaz enzimi gelmektedir. Çalışmada bakteriyel kaynaklı lipaz enziminin, ısıl işleme alternatif gıda muhafaza tekniklerinden biri olan vurgulu elektrik alan uygulaması ile etkinliği araştırılmıştır.
dc.description Milk is an ideal food for microorganisms and enzymes with its high water content, neutral pH and nutritious ingredients. Heat treatment is the best known preservation method to purify raw milk from microorganisms and inactivate enzymes that cause undesirable defects and also to extend the shelf life of milk. Natural enzymes in milk are inactive at pasteurization temperature, but bacterial enzymes are resistant to this temperature. High degree of heat treatment norms cause both nutritional losses and texture disorders in milk. At the same time, the formation of psychrophilic and mesophilic bacteria-derived enzymes resistant to heat treatment occurs with long-term storage. Lipase enzyme, which causes various textural and technological problems in milk technology, comes first among these enzymes. In the study, the effectiveness of bacterial lipase enzyme with pulsed electric field application, which is one of the alternative food preservation techniques to heat treatment, was investigated.
dc.format application/pdf
dc.language tr
dc.publisher Süleyman Demirel Üniversitesi
dc.publisher Süleyman Demirel University
dc.relation https://dergipark.org.tr/tr/download/article-file/1562693
dc.source Volume: 9, Issue: 3 1039-1044 en-US
dc.source 1308-6693
dc.source Mühendislik Bilimleri ve Tasarım Dergisi
dc.subject Vurgulu Elektrik Alan,Mikrobiyal Lipaz Aktivitesi,Psikrotrof Bakteri,Süt
dc.subject Pulsed Electric Field,Microbial Lipase Activity,Psychrotroph Bacteria,Milk
dc.title VURGULU ELEKTRİK ALAN (PEF) TEKNİĞİNİN ÇİĞ SÜTTE MİKROBİYAL LİPAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİNLİĞİNİN ARAŞTIRILMASI tr-TR
dc.title INVESTIGATION OF THE EFFECTIVENESS OF THE PULSED ELECTRIC FIELD (PEF) TECHNIQUE ON MICROBIAL LIPASE ENZYME ACTIVITY IN RAW MILK en-US
dc.type info:eu-repo/semantics/article
dc.citation Ajmal, M., Nadeem, M., Imran, M., & Junaid, M., 2018. Lipid compositional changes and oxidation status of ultra-high temperature treated Milk. Lipids in health and disease, 17(1), 1-11.
dc.citation Alirezalu, K., Munekata, P. E., Parniakov, O., Barba, F. J., Witt, J., Toepfl, S., Lorenzo, J. M., 2020. Pulsed electric field and mild heating for milk processing: a review on recent advances. Journal of the Science of Food and Agriculture, 100(1), 16-24.
dc.citation Bendicho, S., Estela, C., Giner, J., Barbosa-Cánovas, G. V., & Martin, O., 2002. Effects of high intensity pulsed electric field and thermal treatments on a lipase from Pseudomonas fluorescens. Journal of dairy science, 85(1), 19-27.
dc.citation Chen, L. D. R. M., Daniel, R. M., & Coolbear, T., 2003. Detection and impact of protease and lipase activities in milk and milk powders. International dairy journal, 13(4), 255-275.
dc.citation Cserhalmi, Z., Sass-Kiss, A., Tóth-Markus, M., & Lechner, N., 2006. Study of pulsed electric field treated citrus juices. Innovative Food Science & Emerging Technologies, 7(1-2), 49-54.
dc.citation De Jonghe V, Coorevits A, Van Hoorde K, Messens W, Van Landschoot A, De Vos P & Heyndrickx M., 2011. Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk. Appl Environ Microbiol 77: 460– 470.
dc.citation Deeth, H. C., & Fitz-Gerald, C. H., 2006. Lipolytic enzymes and hydrolytic rancidity. Advanced Dairy Chemistry, 2, 481–556.
dc.citation Deeth, H. C., 2006. Lipoprotein lipase and lipolysis in milk. International Dairy Journal, 16(6), 555-562.
dc.citation Deeth, H. C., Touch, V., 2000. Methods for detecting lipase activity in milk and milk products. Methods, 5(5), 555.
dc.citation Eneroth, Å., Ahrné, S., Molin, G., 2000. Contamination of milk with Gram-negative spoilage bacteria during filling of retail containers. International Journal of Food Microbiology, 57(1-2), 99-106.
dc.citation Evrendilek, G. A., Zhang, Q. H., Richter, E. R., 2004. Application of pulsed electric fields to skim milk inoculated with Staphylococcus aureus. Biosystems Engineering, 87(2), 137-144.
dc.citation Gehringer, G., 1980. Multiplikation of bacteria during farm storage. In Factor influensing the bacteriologicalquality of raw milk. International Dairy Federation Bulletin, Document 120.
dc.citation Glantz, M., Rosenlöw, M., Lindmark-Månsson, H., Johansen, L. B., Hartmann, J., Höjer, A., ... & Paulsson, M., 2020. Impact of protease and lipase activities on quality of Swedish raw milk. International Dairy Journal, 107, 104724.
dc.citation Gómez, B., Munekata, P. E., Gavahian, M., Barba, F. J., Martí-Quijal, F. J., Bolumar, T., Lorenzo, J. M., 2019. Application of pulsed electric fields in meat and fish processing industries: An overview. Food Research International, 123, 95-105.
dc.citation Hasan, F., Shah, A. A., Hameed, A., 2009. Methods for detection and characterization of lipases: a comprehensive review. Biotechnology advances, 27(6), 782-798.
dc.citation Ho, S. Y., Mittal, G. S., Cross, J. D., 1997. Effects of high field electric pulses on the activity of selected enzymes. Journal of food engineering, 31(1), 69-84.
dc.citation Jaeger, K. E., Ransac, S., Dijkstra, B. W., Colson, C., van Heuvel, M., & Misset, O., 1994. Bacterial lipases. FEMS microbiology reviews, 15(1), 29-63.
dc.citation Krewinkel, M., Baur, C., Kranz, B., von Neubeck, M., Wenning, M., Scherer, S., ... & Fischer, L., 2016. A sensitive and robust method for direct determination of lipolytic activity in natural milk environment. Food analytical methods, 9(3), 646-655.
dc.citation Kumar, A., Parihar, S. S., Batra, N., 2012. Enrichment, isolation and optimization of lipase-producing Staphylococcus sp. from oil mill waste (Oil cake). Journal of Experimental Sciences, 3(8), 26-30.
dc.citation Li, N., Wang, Y., You, C., Ren, J., Chen, W., Zheng, H., & Liu, Z., 2018. Variation in raw milk microbiota throughout 12 months and the impact of weather conditions. Scientific reports, 8(1), 1-10.
dc.citation Machado, S. G., Baglinière, F., Marchand, S., Van Coillie, E., Vanetti, M. C., De Block, J., & Heyndrickx, M., 2017. The biodiversity of the microbiota producing heat-resistant enzymes responsible for spoilage in processed bovine milk and dairy products. Frontiers in microbiology, 8, 302.
dc.citation Mosqueda-Melgar, J., Elez-Martinez, P., Raybaudi-Massilia, R. M., Martin-Belloso, O., 2008. Effects of pulsed electric fields on pathogenic microorganisms of major concern in fluid foods: a review. Critical Reviews in Food Science and Nutrition, 48(8), 747-759.
dc.citation Ohshima, T., Tamura, T., Sato, M., 2007. Influence of pulsed electric field on various enzyme activities. Journal of Electrostatics, 65(3), 156-161.
dc.citation Roy, R. N., 1980. Fluorimetric assay of the activity of extracellular lipases of Pseudomonas fluorescens and Serratia marcescens. Journal of Applied Bacteriology, 49(2), 265-271.
dc.citation Salgado, C. A., Baglinière, F., & Vanetti, M. C. D., 2020. Spoilage potential of a heat-stable lipase produced by Serratia liquefaciens isolated from cold raw milk. LWT, 126, 109289.
dc.citation Samaržija, D., Zamberlin, Š., & Pogačić, T., 2012. Psychrotrophic bacteria and their negative effects on milk and dairy products quality. Mljekarstvo: časopis za unaprjeđenje proizvodnje i prerade mlijeka, 62(2), 77-95.
dc.citation Sampedro, F., Rodrigo, M., Martinez, A., Rodrigo, D., & Barbosa-Cánovas, G. V., 2005. Quality and safety aspects of PEF application in milk and milk products. Critical Reviews in Food Science and Nutrition, 45(1), 25-47.
dc.citation Saraç, N., Boran, R., Ökmen, G., & Aysel, U. Ğ. U. R., 2008. Toprak ve Süt Kökenli Gram Pozitif Bakterilerde Lipaz Üretimi. Research Journal of Biology Sciences, 1(2), 23-28.
dc.citation Scudino, H., Silva, E. K., Gomes, A., Guimarães, J. T., Cunha, R. L., Sant'Ana, A. S., Cruz, A. G., 2020. Ultrasound stabilization of raw milk: Microbial and enzymatic inactivation, physicochemical properties and kinetic stability. Ultrasonics sonochemistry, 67, 105185.
dc.citation Sharma, P., Oey, I., & Everett, D. W. (2014). Effect of pulsed electric field processing on the functional properties of bovine milk. Trends in food science & technology, 35(2), 87-101.
dc.citation Sharma, P., Oey, I., Bremer, P., & Everett, D. W., 2014. Reduction of bacterial counts and inactivation of enzymes in bovine whole milk using pulsed electric fields. International Dairy Journal, 39(1), 146-156.
dc.citation Thomson, C. A., Delaquis, P. J., & Mazza, G., 1999. Detection and measurement of microbial lipase activity: a review. Critical Reviews in Food Science and Nutrition, 39(2), 165-187.
dc.citation Ünal, R. N., Besler, H. T., 2008. Beslenmede sütün önemi. Sağlık Bakanlığı Yayın, 727.
dc.citation Vega-Mercado, H., Powers, J. R., Barbosa-Canovas, G. V., Swanson, B. G., 2001. Effect of added calcium and EDTA on the inactivation of a protease from Pseudomonas fluorescens M3/6 when exposed to pulsed electric fields (pp. 121-134). Lancaster: Technomic Publishing Company, Inc.
dc.citation Versaw, W. K., Cuppett, S. L., Winters, D. D., Williams, L. E., 1989. An improved colorimetric assay for bacterial lipase in nonfat dry milk. Journal of Food Science, 54(6), 1557-1558.
dc.citation Yang, S., Suwal, S., Andersen, U., Otte, J., Ahrné, L., 2020. Effects of pulsed electric field on fat globule structure, lipase activity, and fatty acid composition in raw milk and milk with different fat globule sizes. Innovative Food Science & Emerging Technologies, 102548.
dc.citation Yangılar, F., 2013. Süt ve Süt Ürünlerinde Hidroksimetilfurfural HMF. Akademik Gıda, 11(3), 70-76.
dc.citation Yangılar, F., Kabil, E., Yılmaz, F., 2013. Pef işleminin süt ve süt ürünlerinde uygulanabilirliği. Manas Journal of Engineering, 1(1), 43-50.
dc.citation Zhang, D., Palmer, J., Teh, K. H., Flint, S., 2020. Identification and selection of heat-stable protease and lipase-producing psychrotrophic bacteria from fresh and chilled raw milk during up to five days storage. LWT, 134, 110165.
dc.citation Zhang, Q., Chang, F. J., Barbosa-Cánovas, G. V., & Swanson, B. G., 1994. Inactivation of microorganisms in a semisolid model food using high voltage pulsed electric fields. LWT-Food Science and Technology, 27(6), 538-543.


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account