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Analysis of the high frequency conjugal transfer systems in lactococci

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dc.creator Tukel, C
dc.creator Akcelik, M
dc.creator Tuncer, Y
dc.date 2005-01-01T01:00:00Z
dc.date.accessioned 2021-12-03T11:46:33Z
dc.date.available 2021-12-03T11:46:33Z
dc.identifier a65b9b96-0c1f-4d45-bf26-64710508f44f
dc.identifier https://avesis.sdu.edu.tr/publication/details/a65b9b96-0c1f-4d45-bf26-64710508f44f/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/93952
dc.description High frequency conjugal transfer systems were analysed in lactococci using by 5 donor and two recipient strains with different phenotypic characters. High frequency transfer of plasmids was found to be associated with the formation of aggregation structures in donor-recipient strain mixtures in the conjugation experiments. Aggregation abilities of Lactococcus lactis strain mixtures were determined to be controlled with a two element-system one of which was encoded by lactose plasmids (Clu) and the other by chromosomal DNA (Agg). Conjugal transfer frequencies of plasmids were obtained at high levels such as 4.2 x 10(-2)-1.5 x 10(-4) per donor cell when aggregate forming donor-recipient mixtures were used. Plasmid stabilities were determined between 82-98 % in conjugants obtained from conjugation experiments in which L. lactis subsp. lactis MLL40-29 was used as recipient. A reduction in the production of a 48.9 kDa protein was determined with the disappearance of the clumping phenotype.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Analysis of the high frequency conjugal transfer systems in lactococci
dc.type info:eu-repo/semantics/article


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