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In vitro studies on callus induction and shoot regeneration from leaf explants of Prunus avium L. rootstock 'F12/1'

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dc.creator Canli, F. A.
dc.creator Demir, F.
dc.date 2016-03-01T01:00:00Z
dc.date.accessioned 2021-12-03T11:46:37Z
dc.date.available 2021-12-03T11:46:37Z
dc.identifier a76d6681-0124-4690-8ca3-5c9afc96b24b
dc.identifier 10.5958/0974-0112.2016.00030.x
dc.identifier https://avesis.sdu.edu.tr/publication/details/a76d6681-0124-4690-8ca3-5c9afc96b24b/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/93979
dc.description To obtain regeneration from leaf explant of 'F12/1' (Prunus avium L.) rootstock, young leaves from 4-week-old in vitro shoot cultures were wounded by transverse cuts three times along the midrib and incubated with abaxial side facing up on Woody Plant Medium (WPM). Shoot regeneration was obtained with benzyladenine (BA) both in dark incubated cultures (5% at 2.5 mu M) and non-dark incubated cultures (5% at 10 mu M). Thidiazuron (TDZ) containing medium also yielded shoot regeneration (5% at 9.08 mu M) in cultures that were kept in continuous light. Overall, TDZ pre-treatment and dark incubations improved callus formation frequencies and callus colony numbers. Regenerates were rooted on WPM containing 9.08 mu M IBA, acclimatized and then transferred to greenhouse conditions.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title In vitro studies on callus induction and shoot regeneration from leaf explants of Prunus avium L. rootstock 'F12/1'
dc.type info:eu-repo/semantics/article


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