| dc.creator |
ŞİRİNYILDIZ, Ferhat; ADNAN MENDERES UNIVERSITY, FACULTY OF MEDICINE |
|
| dc.creator |
CESUR, Gökhan; ADNAN MENDERES UNIVERSITY, FACULTY OF MEDICINE |
|
| dc.date |
2021-08-22T00:00:00Z |
|
| dc.date.accessioned |
2021-12-03T11:46:58Z |
|
| dc.date.available |
2021-12-03T11:46:58Z |
|
| dc.identifier |
https://dergipark.org.tr/tr/pub/sdutfd/issue/64600/891576 |
|
| dc.identifier |
10.17343/sdutfd.891576 |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/94174 |
|
| dc.description |
ObjectiveIschemia reperfusion (IR) injury is caused by a suddenand temporary impairment of blood flow to a particularorgan or tissue. Renal IR-induced kidney damage causeshigh levels of permanent damage and mortality.Dietary omega-3 fatty acids have anti-inflammatory and immune-modulating effects and are used as protectiveagents.Materials and MethodsIn the study; Protective effects of different doses ofomega-3 fatty acid in ischemia reperfusion injury wereinvestigated by biochemical and histological analyzes.40 adult female rats were divided into 5 groupsas sham control, IR, IR+100, IR+400, IR+700 (n:8).The right kidney was removed by nephrectomy in thesham control group, and the left kidney was not treated.For IR injury; the right kidney was removed bynephrectomy, and the left kidney was clamped. Omega-3 fatty acids of 100 mg/kg, 400 mg/kg and 700 mg/kg were administered by gastric gavage to the treatmentgroups for 14 days before the surgery and for15. gastric gavage was administrated in the morningof the operation.ResultsAccording these data; with application of omega-3,histopathological examination results were correlated.It is found that, histopathological results weresupported by biochemical results. There was a significantdecrease in malondialdehyde (MDA) and myeloperoxidase(MPO) levels in the treated groups, whilecatalase (CAT) and glutathione peroxidase (GSH-Px)levels were detected to be increased.ConclusionIt was concluded that administration of omega-3 fattyacids beforehand in cases that may cause ischemiaand reperfusion damage in the kidney can provide aprotective effect on this tissue. |
|
| dc.description |
Amaçİskemi reperfüzyon (IR) hasarı, belirli bir organa kanakışının ani ve geçici olarak bozulmasından kaynaklanır.Renal IR kaynaklı böbrek hasarı, yüksek düzeydekalıcı hasar ve mortaliteye neden olur. Diyet ilealınan omega-3 yağ asitleri, antiinflamatuar ve immünmodüle edici etkilere sahiptir ve koruyucu olarak kullanılmaktadır.Çalışmada; iskemi reperfüzyon hasarındaomega-3 yağ asidinin farklı dozlarının koruyucuetkileri biyokimyasal ve histolojik analizler ile incelenmiştir.Gereç ve Yöntem40 adet erişkin dişi sıçan Düz kontrol, sham kontrol,IR, IR+100, IR+400, IR+700 olmak üzere 5 gruba ayrıldı(n:8). Sham grubunda sağ böbrek nefrektomi ilealındı, sol böbreğe işlem uygulanmadı. I/R hasarınamaruz kalan sağ böbrek nefrektomi ile alındı, sol böbreklereise klemp takıldı. Tedavi gruplarına cerrahi girişimdenönceki 14 gün ve operasyon gününün sabahındatoplam 15 sefer 100 mg/kg, 400 mg/kg ve 700mg/kg’lık omega-3 yağ asitleri gastrik gavaj ile verildi.BulgularElde edilen verilere göre; artan dozlardaki omega-3ile histopatolojik inceleme sonuçları korale şekilde iyileşmetespit edildi. Histopatolojik sonuçlar, biyokimyasalsonuçlar ile desteklendi. Malondaildehit (MDA) vemyeloperoksidaz (MPO) düzeylerinde tedavi uygulanangruplarda anlamlı düzeyde azalma saptanırken,katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) düzeylerindeartış saptandı.SonuçSonuç olarak; elde edilen bulgulara göre, böbrekteiskemi ve reperfüzyon hasarı oluşturma ihtimali olanvakalarda önceden omega-3 yağ asidi verilmesinin budokuda koruyucu etki sağlayabileceği sonucuna ulaşılmıştır. |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
Süleyman Demirel Üniversitesi |
|
| dc.publisher |
Süleyman Demirel University |
|
| dc.relation |
https://dergipark.org.tr/tr/download/article-file/1619426 |
|
| dc.source |
Volume: 28, Issue: 2
283-289 |
en-US |
| dc.source |
1300-7416 |
|
| dc.source |
2602-2109 |
|
| dc.source |
SDÜ Tıp Fakültesi Dergisi |
|
| dc.subject |
Inflammation,Ischemia-Reperfusion Injury,Kidney,Omega 3 fatty acids,Oxidative stress |
|
| dc.subject |
Böbrek,İnflamasyon,İskemi-Reperfüzyon Hasarı,Oksidatif stres,Omega 3 yağ asitleri |
|
| dc.title |
INVESTIGATION OF PROTECTIVE EFFECTS OF DIFFERENT DOSES OF OMEGA 3 FATTY ACIDS IN EXPERIMENTAL RENAL ISCHEMIA REPERFUSION DAMAGE |
en-US |
| dc.title |
DENEYSEL BÖBREK İSKEMİ REPERFÜZYON HASARINDA FARKLI DOZLARDAKİ OMEGA 3 YAĞ ASİTLERİNİN KORUYUCU ETKİLERİNİN ARAŞTIRILMASI |
tr-TR |
| dc.type |
info:eu-repo/semantics/article |
|
| dc.citation |
1. Jang HR, Rabb H. The innate immune response in ischemic acute kidney injury. Clin Immunol. 2009;130:41-50. |
|
| dc.citation |
2. Ergün O, Ulman C, Kılıçalp AS. Carnitine as a agent in experimental renal ischemia-reperfusion injury. UroI Res 2001;29:186-9. |
|
| dc.citation |
3. Guyton AC. The kidneys and body fluids. In: Textbook of Medical Physiology. 9th edition. Philadelphia: WB Saunders Company; 1996. 297–313. |
|
| dc.citation |
4. Gasanov, F., Aytac, B., & Vuruskan, H. (2011). The effects of tadalafil on renal ischemia reperfusion injury: an experimental study. Bosnian journal of basic medical sciences, 11(3), 158. |
|
| dc.citation |
5. Malek, M., & Nematbakhsh, M. (2015). Renal ischemia/reperfusion injury; from pathophysiology to treatment. Journal of renal injury prevention, 4(2), 20. |
|
| dc.citation |
6. Islam CF, Mathie RT, Dinneen MD, Kiely EA, Peters AM, Grace PA. Ischemia–reperfusion injury in the rat kidney the effect of preconditioning. Br J Urol 1997;6:842–7. |
|
| dc.citation |
7. Calder, P. C., & Yaqoob, P. Understanding omega-3 polyunsaturated fatty acids. Postgraduate medicine, 121(6), 148-157.Calder PC: Polyunsaturated fatty acids, inflammation, and immunity. Lipids 2001, 36:1007–1024. |
|
| dc.citation |
8. Mori TA, Beilin LJ: n-3 Fatty acids, blood lipids and cardiovascular risk reduction. Curr Opin Lipidol 2001, 12:1211–1217. |
|
| dc.citation |
9. Alejandro V, Scandling JD, Sibley RK, et al: Mechanisms of filtration failure during postischemic injury of the human kidney. J Clin invest 95: 820-831,1995 |
|
| dc.citation |
10. Simmons MN, Schreiber MJ, Gill IS. Surgical Renal Ischemia: A Contemporary Overview. J Urol. 2008;180(1):19-30. |
|
| dc.citation |
11. Fouad AA, Al-Mulhim AS, Jresat I, Morsy MA. Protective effects of captopril in diabetic rats exposed to ischemia/reperfusion renal injury. J Pharm Pharmacol. 2013;65(2):243- 252. |
|
| dc.citation |
12. Kocoglu H, Ozturk H, Ozturk H, et al. Effect of dexmedetomidine on ischemiareperfusion injury in rat kidney: A histopathologic study. Ren Fail. 2009;31(1):70-74. |
|
| dc.citation |
13. Williams P, Lopez H, Britt D, et al. Characterization of Renal Ischemia-Reperfusion Injury in Rats. J Pharmacol Toxicol Methods. 1997;Feb:37(1):1-7 |
|
| dc.citation |
14. Mortensen M, Rosenfield RL, Littlejohn E. Functional significance of polycysticsize ovaries in healthy adolescents. J Clin Endocrinol Metab, 2006, 91: 3786-3790. |
|
| dc.citation |
15. Sarsılmaz M, Songur A, Özyurt H, et al. Potential role of dietary ω-3 essential fatty acids on some oxidant/antioxidant parameters in rats’ corpus striatum. Prostagl Leukot Essent Fatty Acids 2003; 69(4): 253-259. |
|
| dc.citation |
16. Miyasaca CK, Alves de Souza JA, Torres RP, Filho JM, Lajolo FM, Curi R. Effect of the administration of fish oil by gavage on activities of antioxidant enzymes of rat lympohoid organs. Gen Pharmacol 1998; 30 (5): 759-762. |
|
| dc.citation |
17. Grande JP, Walker HJ, Holub BJ ve ark. Suppressive effects of fish oil on mesengial cell proliferation in vitro and in vivo.Kidney Int 2000;57:1027-1040 |
|
| dc.citation |
18. Slater TF. Free radical mechanisms in tissue injury. J Biochem 1984; 222:1-15. |
|
| dc.citation |
19. Ozan E. Koyutürk L.Sapmaz T. Böbrek İskemi Reperfüzyon Hasarında Antioksidan Olarak Prostoglandin E1(PGE)Kullnımının İncelenmesi: Fırat Üniversitesi Tıp Fakültesi Histoloji Ve Embriyoloji Anabilimdalı. Fırat Tıp Dergisi 2004;9(3):67-71 |
|
| dc.citation |
20. Ohkawa H, Ohishi N, Tagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-358. |
|
| dc.citation |
21. İnal M, Altınışık M, Bilgin MD. The effect of quercetin on renal ischemia and reperfusion injury in the rat. Cell Biochem Funct. 2002 dec; 20(4):291-6. |
|
| dc.citation |
22. Wall J. Antioxidants In Prevention Of Reperfusion Damage Of Vascular Endothelium. TSMJ May 2000;1:67-70. |
|
| dc.citation |
23. Becker BF, Reinholz N, Leipert B, Raschke P, Permanetter B, Gerlach E. Role of uric acid as an endogenous radical scavenger and antioxidant. Chest. 1991; 100: 176-181. |
|