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<h3 class="c-article__sub-heading" data-test="abstract-sub-heading" style="box-sizing: inherit; margin: 0px 0px 8px; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Oxygen-Sans, Ubuntu, Cantarell, "Helvetica Neue", sans-serif; line-height: 1.24; color: rgb(34, 34, 34); letter-spacing: normal; background-color: rgb(252, 252, 252);">Background</h3><p style="box-sizing: inherit; padding: 0px; margin-bottom: 1.5em; overflow-wrap: break-word; line-height: 1.8; color: rgb(51, 51, 51); font-family: Georgia, Palatino, serif; font-size: 18px; background-color: rgb(252, 252, 252);">We investigated the apoptotic effects of curcumin in the colon carcinoma cell line SW480.</p><h3 class="c-article__sub-heading" data-test="abstract-sub-heading" style="box-sizing: inherit; margin: 0px 0px 8px; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Oxygen-Sans, Ubuntu, Cantarell, "Helvetica Neue", sans-serif; line-height: 1.24; color: rgb(34, 34, 34); letter-spacing: normal; background-color: rgb(252, 252, 252);">Methods and results</h3><p style="box-sizing: inherit; padding: 0px; margin-bottom: 1.5em; overflow-wrap: break-word; line-height: 1.8; color: rgb(51, 51, 51); font-family: Georgia, Palatino, serif; font-size: 18px; background-color: rgb(252, 252, 252);">Cells were treated with 40–200 μM curcumin for 24, 48, and 72 h, and the IC<span style="box-sizing: inherit; font-size: 13.5px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;">50</span> values were determined for each time interval. BrdU, caspase-3, and TUNEL staining results and the gene expression of <i style="box-sizing: inherit;">FADD</i>, <i style="box-sizing: inherit;">CASP8</i>, and <i style="box-sizing: inherit;">CASP3</i> were evaluated. Curcumin treatments significantly inhibited cell proliferation and significantly induced apoptosis for 24, 48, and 72 h. The proportion of BrdU-stained cells in the control groups were 58%, 57% and 61% and 28%, 27%, and 30% in the curcumin treatment groups at 24, 48, and 72 h, respectively. The proportion of apoptotic cells was 28%, 29%, and 28% in the control groups and 59%, 61%, and 60% in the curcumin treatment groups at 24, 48, and 72 h, respectively. As expected, caspase-3 staining also revealed a higher number of apoptotic cells in curcumin treatment groups at 24, 48, and 72 h compared to controls. The proportion of Caspase-3-stained cells in the control groups were 23%, 25%, and 24% and 59%, 60%, and 62% in the curcumin treatment groups at 24, 48, and 72 h, respectively. To prove caspase-3 staining results, <i style="box-sizing: inherit;">FADD</i>, <i style="box-sizing: inherit;">CASP8</i>, and <i style="box-sizing: inherit;">CASP3</i> gene expressions were evaluated by real-time qPCR. Unlike the immunohistochemical results, no statistically significant upregulation was found at 24 and 48 h, while relative gene expressions of <i style="box-sizing: inherit;">FADD</i>, <i style="box-sizing: inherit;">CASP8</i>, and <i style="box-sizing: inherit;">CASP3</i> was significantly upregulated at 72 h. The expression level increase was 0.88-, 1.19-, and 2.11-fold for <i style="box-sizing: inherit;">FADD</i>, 1.25-, 1.29-, and 1.59-fold for <i style="box-sizing: inherit;">CASP8</i>, and 1.33-, 1.46-, and 3.00-fold for <i style="box-sizing: inherit;">CASP3</i> at 24, 48, and 72 h, respectively.</p><h3 class="c-article__sub-heading" data-test="abstract-sub-heading" style="box-sizing: inherit; margin: 0px 0px 8px; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Oxygen-Sans, Ubuntu, Cantarell, "Helvetica Neue", sans-serif; line-height: 1.24; color: rgb(34, 34, 34); letter-spacing: normal; background-color: rgb(252, 252, 252);">Conclusions</h3><p style="box-sizing: inherit; padding: 0px; margin-bottom: 1.5em; overflow-wrap: break-word; line-height: 1.8; color: rgb(51, 51, 51); font-family: Georgia, Palatino, serif; font-size: 18px; background-color: rgb(252, 252, 252);">These results suggest that curcumin may be a potential protective or treatment agent against colon cancer; however, further studies on curcumin-rich diets and curcumin bioavailability are required.</p> |
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