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In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars

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dc.creator Canli, F. A.
dc.creator Tian, L.
dc.date 2008-03-10T01:00:00Z
dc.date.accessioned 2021-12-03T12:04:42Z
dc.date.available 2021-12-03T12:04:42Z
dc.identifier ecb3e25a-636a-469c-8816-7a28351be0bb
dc.identifier 10.1016/j.scienta.2007.10.023
dc.identifier https://avesis.sdu.edu.tr/publication/details/ecb3e25a-636a-469c-8816-7a28351be0bb/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/95668
dc.description Shoot regeneration was achieved from stored mature cotyledons of sweet cherry (Prunus avium L.) in vitro. The influences of different cytokinins (thidiazuron (TDZ) and benzylaminopurine (BA)) and their levels, the dark incubation for the first 10 days of the culture and TDZ pretreatments on shoot regeneration were determined. All varieties regenerated in the presence of TDZ if cotyledons were maintained in darkness for the first 10 days of the culture; however, only three varieties regenerated with low frequencies in the absence of dark incubation. Dark incubation at the early stage of culture was critical for obtaining higher regeneration efficiencies from stored cherry cotyledons. TDZ was more effective than BA in inducing shoot regeneration. The highest regeneration efficiencies were obtained with intermediate concentrations of TDZ (3.6 and 7.2 mu M) in combination with dark incubation and the best regeneration frequencies for 'Vista', 'Sunburst', 'Tehranivee', Nouge' and 'Heidelfingen' cotyledons were 70.0%, 53.3%, 23.3%, 30% and 26.6%, respectively. (c) 2007 Elsevier B.V. All rights reserved.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars
dc.type info:eu-repo/semantics/article


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