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THE EFFECTS OF HUMAN AMNIOTIC FLUID AND MEMBRANE ON CHONDRAL HEALING IN A RABBIT KNEE CARTILAGE DEFECT MODEL

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dc.creator SATOĞLU, İsmail Safa; DOKUZ EYLUL UNIVERSITY
dc.creator UNAL, Meric; SÜLEYMAN DEMİREL ÜNİVERSİTESİ
dc.creator ÇOBAN, İbrahim; Özel Adatıp Hastanesi
dc.creator GÜREL, Duygu; DOKUZ EYLÜL ÜNİVERSİTESİ
dc.creator GÜLTEKİN, Alper; KOCAELİ DERİNCE EĞİTİM VE ARAŞTIRMA HASTANESİ
dc.creator TURGUT, Necmettin; ISPARTA ŞEHİR HASTANESİ
dc.creator KARAOĞLAN, Osman; DOKUZ EYLUL UNIVERSITY
dc.date 2021-12-30T00:00:00Z
dc.date.accessioned 2022-05-10T10:58:54Z
dc.date.available 2022-05-10T10:58:54Z
dc.identifier https://dergipark.org.tr/tr/pub/sdutfd/issue/67913/830427
dc.identifier 10.17343/sdutfd.830427
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/96208
dc.description ObjectiveDue to the limited intrinsic healing and repair capacityof the articular cartilage, most treatment methodscannot achieve reliable regeneration of normalhyaline cartilage, resulting in early developmentof osteoarthritis. The purpose of this study was todetermine the effects of human amniotic fluid andmembrane on chondral defects.Material and MethodsSixty-four knees of 32 immature New Zealand rabbitswere included in the study. Full thickness chondraldefects were created in the weight-bearing surface ofthe medial condyles of the rabbits. The rabbits weredivided randomly into four groups: no adjunct treatmentwas given in group 1, 0.3 ml human amniotic fluid(HAF) alone in group 2, human amniotic membrane(HAM) alone in group 3 and both of 0.3 ml HAF andHAM in group 4 was administered. The condyles werehistopathologically evaluated at 4th and 12th weekusing the modified O’Driscoll Grading Scale.ResultsThere were no significant differences in the qualityof the regenerated tissue within and between groups(p>0.05). The mean results of groups at the 12th weekwere worse than results at the 4th week; however,the difference was statistically significant for only thesham group (group 1) and the combined therapygroup (group 4) (p=0.007 and p=0.014, respectively).ConclusionHAF alone, HAM alone, and combined administrationof both biomaterials neither affected chondral defecthealing nor had any differences between eachother. Nevertheless, we believe that some earlyregeneration due to an intrinsic repair mechanism ispossible in immature rabbits as this study showedbetter results at 4th week than those at 12th week,although they are prone to degenerative processes inlong-term follow-up. We suggest that a larger samplesize in an experimental study would probably displaya statistically significant difference when investigatingeffects of HAF, HAM, or both.
dc.description AmaçArtiküler kıkırdağın kısıtlı intrensek iyileşmesi ve tamirkapasitesinin az olması nedeniyle çoğu tedavimetodları ile normal hyalen kıkırdak rejenerasyonusağlanamamakta ve sonuç olarak osteoartroz gelişebilmektedir.Bu çalışmada insan amniyotik sıvısı vemembranının tavşanlardaki kondral defektlerdeki etkileriniincelemek amaçlanmıştır.Gereç ve Yöntem32 immatür albino Yeni Zelanda tavşanının 64 dizi buçalışmaya dahil edildi. Tam kat kıkırdak defektleri tavşanlarınmedial kondillerinin yük taşıma yüzeylerindecerrahi olarak oluşturuldu. Tavşanlar randomize olarakdört gruba bölündü: ek tedavi verilmeyenler Grup1, 0,3 ml insan amniyotik sıvısı (İAS) verilenler Grup2, sadece insan amniyotik membranı (İAM) verilenlerGrup 3, hem 0,3 ml İAS hem İAM verilenler Grup4’ü oluşturdu. Kondiller histopatolojik olarak 4. ve 12.haftalarda modifiye O’Driscoll evreleme skalası iledeğerlendirildi. Sonuçlar Mann-Whitney U ve ANOVAtestleri ile istatistiksel olarak analiz edildi.BulgularGruplar arasında rejenere dokunun kalitesi açısındanistatistiksel olarak anlamlı fark bulunmadı (p>0.05).Grupların ortalama sonuçları 12. haftada, 4. hafta sonuçlarındandaha kötü olarak saptandı; buna rağmenbu farkın, sadece Grup 1 (sham grubu) ve Grup 4 açısındananlamlı olduğu görüldü (İAS+İAM) (sırasıyla,p=0,007 ve p=0,014).SonuçSadece İAS, sadece İAM ve her iki biyomateryalinkombine edildiği tedavi yöntemlerinin hiçbirinde kıkırdakdefekt iyileşmesi daha iyi ve kaliteli olmadığıve birbirlerine de bir üstünlüğü olmadığı görüldü. Buçalışmada gösterilmiş olan 4. hafta sonuçlarının 12.hafta sonuçlarından daha iyi olmasının çalışmadakullanılan immatür tavşanlardaki intrensek tamir mekanizmasındakierken rejenerasyon kapasitesindenkaynaklandığını düşünmekteyiz.
dc.format application/pdf
dc.language en
dc.publisher Süleyman Demirel Üniversitesi
dc.publisher Süleyman Demirel University
dc.relation https://dergipark.org.tr/tr/download/article-file/1412212
dc.source Volume: 28, Issue: 4 663-671 en-US
dc.source 1300-7416
dc.source 2602-2109
dc.source SDÜ Tıp Fakültesi Dergisi
dc.subject hyaline cartilage,cartilage regeneration,chondral defect,human amniotic fluid,human amniotic membrane
dc.subject hyalen kıkırdak,kıkırdak rejenerasyonu,kondral defekt,insan amniyotik sıvısı,insan amniyotik membranı
dc.title THE EFFECTS OF HUMAN AMNIOTIC FLUID AND MEMBRANE ON CHONDRAL HEALING IN A RABBIT KNEE CARTILAGE DEFECT MODEL en-US
dc.title İNSAN AMNİYOTİK SIVISI VE MEMBRANININ BİR TAVŞAN DİZİ KIKIRDAK DEFEKTİ MODELİNDE KONDRAL İYİLEŞME ÜZERİNE ETKİLERİ tr-TR
dc.type info:eu-repo/semantics/article
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