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PROTECTIVE EFFECT OF THE RAMELTEON, A MELATONIN AGONIST, AGAINST METHOTREXATE-INDUCED BONE-TOXICITY

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dc.creator DİNÇER, Recep; Süleyman Demirel Üniversitesi Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı
dc.creator BAYKAL, Tuba; SULEYMAN DEMIREL UNIVERSITY
dc.creator KUMBUL DOĞUÇ, Duygu; SULEYMAN DEMIREL UNIVERSITY
dc.creator SARMAN, Emine; SULEYMAN DEMIREL UNIVERSITY
dc.creator CEYLAN, Devran; SULEYMAN DEMIREL UNIVERSITY
dc.date 2022-03-01T00:00:00Z
dc.date.accessioned 2022-05-10T10:59:01Z
dc.date.available 2022-05-10T10:59:01Z
dc.identifier https://dergipark.org.tr/tr/pub/sdutfd/issue/68474/979473
dc.identifier 10.17343/sdutfd.979473
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/96239
dc.description ObjectiveMethotrexate (MTX) used anti-metabolite, causesbone morbidity, including growth arrest and reducedbone mineral density. Melatonin, produced by thepineal gland, has also multiple positive effects in humanbone cells, and positive effects on bone. Ramelteon(RMT) is a non-selective melatonin receptor agonist.In this study, we investigated whether ramelteon, amelatonin agonist, has a protective effect on MTXinducedbone toxicity.Material and MethodsThe rats divided into 4 groups, including Group 1control group; Group 2 MTX group (20 mg/kg); Group3 MTX+RMT (20 mg/kg + 10 mg/kg); Group 4 RMT (10mg/kg). Oral ramelteon and intraperitoneal mtx wereapplied to the rats on the second day according tothe groups. After 7 days, long bones were evaluatedhistologically with hematoxylin-eosin (HE) staining andimmunohistochemically with Catepsin K and RUN X2staining. For statistical analysis immunohistochemicalscores of the groups were compared between thegroups for this purpose, the Oneway ANOVA Duncantest was used by SPSS-22.00 package program.ResultsThere was no significant difference between thecontrol group (group I) and the experimental groups(group II-group III-group IV) in H&E staining of bonetissue sections (p>0.05). No positive staining wasobserved in any of the groups in CAT-K and RUN-Ximmunostaining (p>0.05).ConclusionIt was showed that ramelteon has no anabolicfunction in bone turnover, histopathological andimmunohistochemical, in bone toxicity induced byhigh-dose methotrexate on intact bone tissue.
dc.description AmaçMetotreksat (MTX) büyüme durması ve kemik mineralyoğunluğunun azalması dahil olmak üzere kemikmorbiditesine neden olan anti-metabolittir. Epifiz bezitarafından üretilen melatonin, insan kemik hücrelerindede birçok olumlu etkiye ve kemik üzerinde olumluetkilere sahiptir. Ramelteon (RMT), seçici olmayanbir melatonin reseptör agonistidir. Bu çalışmada, birmelatonin agonisti olan ramelteonun MTX kaynaklıkemik toksisitesi üzerinde koruyucu bir etkisinin olupolmadığını araştırdık.Gereç ve YöntemSıçanlar Grup 1 kontrol grubu olmak üzere 4 gruba ayrıldı;Grup 2 MTX grubu (20 mg/kg); Grup 3 MTX+RMT(20 mg/kg + 10 mg/kg); Grup 4 RMT (10 mg/kg).Ratlara gruplara göre ikinci gün oral ramelteon veintraperitoneal mtx uygulandı. 7 gün sonra uzun kemiklerhematoksilen-eozin (HE) boyama ile histolojikolarak ve Catepsin K ve RUN X2 boyama ile immünohistokimyasalolarak değerlendirildi. İstatistiksel analiziçin grupların immünhistokimyasal skorları gruplararasında karşılaştırıldı, bu amaçla SPSS-22.00 paketprogramı ile Oneway ANOVA Duncan testi kullanıldı.BulgularKemik doku kesitlerinin H&E boyamasında kontrolgrubu (grup I) ile deney grupları (grup II-grup III-grupIV) arasında anlamlı bir fark yoktu (p>0.05). CAT-Kve RUN-X immün boyamasında grupların hiçbirindepozitif boyanma gözlenmedi (p>0.05).SonuçRamelteonun, sağlam kemik dokusu üzerinde yüksekdoz metotreksatın neden olduğu kemik toksisitesinde,kemik döngüsünde, histopatolojik ve immünohistokimyasalolarak anabolik bir işlevi olmadığı gösterilmiştir.
dc.format application/pdf
dc.language en
dc.publisher Süleyman Demirel Üniversitesi
dc.publisher Süleyman Demirel University
dc.relation https://dergipark.org.tr/tr/download/article-file/1911520
dc.source Volume: 29, Issue: 1 53-58 en-US
dc.source 1300-7416
dc.source 2602-2109
dc.source SDÜ Tıp Fakültesi Dergisi
dc.subject METHOTREXATE,RAMELTEON,MELATONIN,RUNX2,Catepsin K
dc.subject Metotreksat,Ramelteon,Melatonin,Katepsin-K,RUNX2
dc.title PROTECTIVE EFFECT OF THE RAMELTEON, A MELATONIN AGONIST, AGAINST METHOTREXATE-INDUCED BONE-TOXICITY en-US
dc.title MELATONİN AGONİSTİ OLAN RAMELTEONUN METOTREKSAT KAYNAKLI KEMİK TOKSİSİTESİNE KARŞI KORUYUCU ETKİSİ tr-TR
dc.type info:eu-repo/semantics/article
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