| dc.creator |
Celebi, Nevin |
|
| dc.creator |
Pinar, Sıla |
|
| dc.creator |
PEZİK, ESRA |
|
| dc.creator |
MUTLU AĞARDAN, NECİBE BAŞARAN |
|
| dc.date |
2022-01-01T00:00:00Z |
|
| dc.date.accessioned |
2022-05-10T11:15:18Z |
|
| dc.date.available |
2022-05-10T11:15:18Z |
|
| dc.identifier |
111c26bb-d9f1-43a2-ad2a-4bd6cc1c6fc0 |
|
| dc.identifier |
10.1080/10837450.2021.2020817 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/111c26bb-d9f1-43a2-ad2a-4bd6cc1c6fc0/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/96476 |
|
| dc.description |
Cyclosporine A is a calcineurin inhibitor and is usually used as an immunosuppressant medication. The main purpose of this study is to develop nanosuspension of polypeptide cyclosporine A by using the wet milling method for oral administration. Cell culture studies were also performed with human intestinal Caco-2 cell lines. Hydroxypropyl methylcellulose and sodium dodecyl sulfate were used as stabilizers in nanosuspension. In vitro characterization studies such as Fourier-transform infrared analysis and morphological imaging with scanning electron microscopy have been carried out with obtained cyclosporine A nanosuspension. The particle size, particle size distribution, and zeta potential values of the nanosuspension were measured approximately 400 nm, 0.4, and -25 mV, respectively. The solubility of cyclosporine A was increased 4.5 times in nanosuspension compared to the coarse cyclosporine A powder. As a result of cytotoxicity studies conducted with different concentrations, it was decided to conduct permeability studies at a dose equivalent to 150 mu g/mL cyclosporine A. Permeation studies have shown that the nanosuspension increases cyclosporine A transport by 5 and 1.5 times, respectively, compared to coarse powder and commercial product. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Development of cyclosporine A nanosuspension: cytotoxicity and permeability on Caco-2 cell lines |
|
| dc.type |
info:eu-repo/semantics/article |
|