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Melatonin Metabolites Protect Human Retinal Pigment Epithelial Cells from Death Caused by Oxidative Stress

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dc.creator Hakan, Kurt Akif
dc.creator Derya, Kilicaslan
dc.creator Ebru, Uras
dc.creator Adem, Doganer
dc.creator Bilge, Alli
dc.date 2021-11-01T00:00:00Z
dc.date.accessioned 2022-05-10T11:17:04Z
dc.date.available 2022-05-10T11:17:04Z
dc.identifier 3db5796d-bbc8-4b67-966f-fbf52843f681
dc.identifier 10.1007/s11094-021-02490-1
dc.identifier https://avesis.sdu.edu.tr/publication/details/3db5796d-bbc8-4b67-966f-fbf52843f681/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/96574
dc.description This study was aimed to determine the efficacy of 6-hydroxy melatonin and N-acetyl serotoninin protecting human retinal pigment epithelial cells from death brought about by oxidative-stress. The ARPE-19 cells (one of the retinal pigment epithelial cell lines) were used as the test cell culture. Following the application of 500 mu M H2O2, ARPE-19 cell line culture was incubated for 20 h to induce oxidative stress damage. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay was used to evaluate the oxidative injury to ARPE-19 cells caused by H2O2 and the effectiveness of 6-hydroxy melatonin and N-acetyl serotonin application in preventing this damage. The results indicated that 6-hydroxy melatonin and N-acetyl serotonin exhibited a therapeutic effect on cells damaged by exposure to oxidative stress. It was concluded that these melatonin metabolites might be useful in treating many retinal conditions linked to the oxidative stress-induced damage. In addition, this study proposed a new idea for developing chemical approaches to the design and synthesis/semisynthesis of new drugs.
dc.language eng
dc.rights info:eu-repo/semantics/closedAccess
dc.title Melatonin Metabolites Protect Human Retinal Pigment Epithelial Cells from Death Caused by Oxidative Stress
dc.type info:eu-repo/semantics/article


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