| dc.creator |
Vatansever, Buse |
|
| dc.creator |
Ozturk, Kuyas Hekimler |
|
| dc.creator |
AKGÜN, EGEMEN |
|
| dc.creator |
İÇDUYGU, FADİME MUTLU |
|
| dc.creator |
ŞAMLI, HALE |
|
| dc.creator |
Ozgoz, Asuman |
|
| dc.date |
2021-10-01T00:00:00Z |
|
| dc.date.accessioned |
2022-05-10T11:19:17Z |
|
| dc.date.available |
2022-05-10T11:19:17Z |
|
| dc.identifier |
73596663-2c85-490d-8dc7-559175f62ea9 |
|
| dc.identifier |
10.17219/acem/140590 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/73596663-2c85-490d-8dc7-559175f62ea9/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/96720 |
|
| dc.description |
Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells |
|
| dc.type |
info:eu-repo/semantics/article |
|