| dc.creator |
Veltman, Douwe M. |
|
| dc.creator |
Firtel, Richard A. |
|
| dc.creator |
van Haastert, Peter J. M. |
|
| dc.creator |
Liu, Youtao |
|
| dc.creator |
Fusetti, Fabrizia |
|
| dc.creator |
Kortholt, Arjan |
|
| dc.creator |
Lacal, Jesus |
|
| dc.date |
2016-06-01T00:00:00Z |
|
| dc.date.accessioned |
2023-01-09T12:01:29Z |
|
| dc.date.available |
2023-01-09T12:01:29Z |
|
| dc.identifier |
36392412-0717-4aaa-b254-e3379fbf7b47 |
|
| dc.identifier |
10.1016/j.devcel.2016.05.001 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/36392412-0717-4aaa-b254-e3379fbf7b47/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/97739 |
|
| dc.description |
Chemotaxis, or directional movement toward extracellular chemical gradients, is an important property of cells that is mediated through G-protein-coupled receptors (GPCRs). Although many chemotaxis pathways downstream of G beta gamma have been identified, few G alpha effectors are known. G alpha effectors are of particular importance because they allow the cell to distinguish signals downstream of distinct chemoattractant GPCRs. Here we identify GflB, a G alpha 2 binding partner that directly couples the Dictyostelium cyclic AMP GPCR to Rap1. GflB localizes to the leading edge and functions as a G alpha-stimulated, Rap1-specific guanine nucleotide exchange factor required to balance Ras and Rap signaling. The kinetics of GflB translocation are fine-tuned by GSK-3 phosphorylation. Cells lacking GflB display impaired Rap1/Ras signaling and actin and myosin dynamics, resulting in defective chemotaxis. Our observations demonstrate that GflB is an essential upstream regulator of chemoattractant-mediated cell polarity and cytoskeletal reorganization functioning to directly link G alpha activation to monomeric G-protein signaling. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
A G alpha-Stimulated RapGEF Is a Receptor-Proximal Regulator of Dictyostelium Chemotaxis |
|
| dc.type |
info:eu-repo/semantics/article |
|