| dc.creator |
Keizer-Gunnink, Ineke |
|
| dc.creator |
van Haastert, Peter J. M. |
|
| dc.creator |
Xu, Xuehua |
|
| dc.creator |
Kortholt, Arjan |
|
| dc.creator |
Jin, Tian |
|
| dc.creator |
Fusetti, Fabrizia |
|
| dc.creator |
Kataria, Rama |
|
| dc.date |
2013-04-01T00:00:00Z |
|
| dc.date.accessioned |
2023-01-09T12:04:10Z |
|
| dc.date.available |
2023-01-09T12:04:10Z |
|
| dc.identifier |
73d895d6-f19f-4087-b97c-0bde67c2ad44 |
|
| dc.identifier |
10.1073/pnas.1301851110 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/73d895d6-f19f-4087-b97c-0bde67c2ad44/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/97996 |
|
| dc.description |
Heterotrimeric G proteins couple external signals to the activation of intracellular signal transduction pathways. Agonist-stimulated guanine nucleotide exchange activity of G-protein-coupled receptors results in the exchange of G-protein-bound GDP to GTP and the dissociation and activation of the complex into G alpha-GTP and a G beta gamma dimer. In Dictyostelium, a basal chemotaxis pathway consisting of heterotrimeric and monomeric G proteins is sufficient for chemotaxis. Symmetry breaking and amplification of chemoattractant sensing occurs between heterotrimeric G protein signaling and Ras activation. In a pull-down screen coupled to mass spectrometry, with G alpha proteins as bait, we have identified resistant to inhibitors of cholinesterase 8 (Ric8) as a nonreceptor guanine nucleotide exchange factor for G alpha-protein. Ric8 is not essential for the initial activation of heterotrimeric G proteins or Ras by uniform chemoattractant; however, it amplifies G alpha signaling, which is essential for Ras-mediated symmetry breaking during chemotaxis and development. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Dictyostelium Ric8 is a nonreceptor guanine exchange factor for heterotrimeric G proteins and is important for development and chemotaxis |
|
| dc.type |
info:eu-repo/semantics/article |
|