| dc.creator |
Bader, Sonya |
|
| dc.creator |
Van Haastert, Peter J. M. |
|
| dc.creator |
Snippe, Helena |
|
| dc.creator |
Kortholt, Arjan |
|
| dc.date |
2006-07-01T00:00:00Z |
|
| dc.date.accessioned |
2023-01-09T12:08:14Z |
|
| dc.date.available |
2023-01-09T12:08:14Z |
|
| dc.identifier |
cf32060d-8f0d-45b5-9938-2f33f9b1031c |
|
| dc.identifier |
10.1074/jbc.m600040200 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/cf32060d-8f0d-45b5-9938-2f33f9b1031c/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/98387 |
|
| dc.description |
Dictyostelium discoideum cells possess multiple cyclic nucleotide phosphodiesterases that belong either to class I enzymes that are present in all eukaryotes or to the rare beta-lactamase class II. We describe here the identification and characterization of DdPDE4, the third class I enzyme of Dictyostelium. The deduced amino acid sequence predicts that DdPDE4 has a leader sequence, two transmembrane segments, and an extracellular catalytic domain that exhibits a high degree of homology with human cAMP-specific PDE8. Expression of the catalytic domain of DdPDE4 shows that the enzyme is a cAMP-specific phosphodiesterase with a Km of 10 mu M; cGMP is hydrolyzed at least 100-fold more slowly. The full-length protein is shown to be membrane-bound with catalytic activity exposed to the extracellular medium. Northern blots and activity measurements reveal that expression of DdPDE4 is low during single cell stages and increases at 9 h of starvation, corresponding with mound stage. A function during multicellular development is confirmed by the phenotype of ddpde4(-) knock-out strains, showing normal aggregation but impaired development from the mound stage on. These results demonstrate that DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/openAccess |
|
| dc.title |
DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells |
|
| dc.type |
info:eu-repo/semantics/article |
|