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Comparative Study on the Effect of Phenolics and Their Antioxidant Potential of Freeze-Dried Australian Beach-Cast Seaweed Species upon Different Extraction Methodologies

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dc.creator AĞAR, Osman Tuncay
dc.creator Suleria, Hafiz A. R.
dc.creator Subbiah, Vigasini
dc.creator Ebrahimi, Faezeh
dc.creator Dunshea, Frank R.
dc.creator Barrow, Colin J.
dc.date 2023-05-01T00:00:00Z
dc.date.accessioned 2025-02-25T10:17:35Z
dc.date.available 2025-02-25T10:17:35Z
dc.identifier 167ecce6-a21c-4b60-9d64-eeb6eb59abd3
dc.identifier 10.3390/ph16050773
dc.identifier https://avesis.sdu.edu.tr/publication/details/167ecce6-a21c-4b60-9d64-eeb6eb59abd3/oai
dc.identifier.uri http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/98888
dc.description Brown seaweed is rich in phenolic compounds and has established health benefits. However, the phenolics present in Australian beach-cast seaweed are still unclear. This study investigated the effect of ultrasonication and conventional methodologies using four different solvents on free and bound phenolics of freeze-dried brown seaweed species obtained from the southeast Australian shoreline. The phenolic content and their antioxidant potential were determined using in vitro assays followed by identification and characterization by LC-ESI-QTOF-MS/MS and quantified by HPLC-PDA. The Cystophora sp. displayed high total phenolic content (TPC) and phlorotannin content (FDA) when extracted using 70% ethanol (ultrasonication method). Cystophora sp., also exhibited strong antioxidant potential in various assays, such as DPPH, ABTS, and FRAP in 70% acetone through ultrasonication. TAC is highly correlated to FRAP, ABTS, and RPA (p < 0.05) in both extraction methodologies. LC-ESI-QTOF-MS/MS analysis identified 94 and 104 compounds in ultrasound and conventional methodologies, respectively. HPLC-PDA quantification showed phenolic acids to be higher for samples extracted using the ultrasonication methodology. Our findings could facilitate the development of nutraceuticals, pharmaceuticals, and functional foods from beach-cast seaweed.
dc.language eng
dc.rights info:eu-repo/semantics/openAccess
dc.title Comparative Study on the Effect of Phenolics and Their Antioxidant Potential of Freeze-Dried Australian Beach-Cast Seaweed Species upon Different Extraction Methodologies
dc.type info:eu-repo/semantics/article


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