| dc.creator |
KUMBUL DOĞUÇ, Duygu |
|
| dc.creator |
ŞAHİN, Uğur |
|
| dc.creator |
Cicek, Emine |
|
| dc.creator |
Temizyurek, Iftihar |
|
| dc.creator |
İLHAN, İlter |
|
| dc.creator |
CALAPOĞLU, Mustafa |
|
| dc.date |
2023-01-01T00:00:00Z |
|
| dc.date.accessioned |
2025-02-25T10:17:41Z |
|
| dc.date.available |
2025-02-25T10:17:41Z |
|
| dc.identifier |
183eb498-ca8a-444e-a565-abfd8a24d122 |
|
| dc.identifier |
10.36721/pjps.2023.36.1.reg.199-204.1 |
|
| dc.identifier |
https://avesis.sdu.edu.tr/publication/details/183eb498-ca8a-444e-a565-abfd8a24d122/oai |
|
| dc.identifier.uri |
http://acikerisim.sdu.edu.tr/xmlui/handle/123456789/98904 |
|
| dc.description |
This study determines obestatin-like substances from the young shoots of the tea plant [Camellia sinensis (L.) O. Kuntze (Theaceae)]. Proteins were extracted from the vegetative tea leaves using the QB (Quick Buffer) buffer as an extraction buffer. Obestatin-like substances in tea extract were investigated using an indirect home-made enzyme-linked immunosorbent assay (ELISA). Human obestatin-like immunoreactive substances from tea extract were isolated and characterized by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) and immunoblotting techniques. Immunochemical results showed that there are strong human obestatin-like immunoreactive substances (0.048 +/- 0.0064 ng/mg protein) in vegetative tea leaves. This finding was completely unexpected since this hormone was considered to be present solely in animals. Furthermore, a single obestatin-like immunoreactive protein band of 13 kDa was identified by tricine-SDS-PAGE and Western blotting of extract of vegetative tea leaf proteins. Present investigation is the first report of presence of obestatin-like immunoreactive substances in plants. It is concluded that obestatin-like bioactive peptides derived from plants can affect gastrointestinal tract structures as endogenous obestatin does and hence play a role in appetite regulation and body weight gain. |
|
| dc.language |
eng |
|
| dc.rights |
info:eu-repo/semantics/closedAccess |
|
| dc.title |
Immunochemical identification of the mammalian peptide hormone obestatin from tea plant (Camellia sinensis) |
|
| dc.type |
info:eu-repo/semantics/article |
|