Objective: There is no study examining the difference of LESS-RT (Landing Error Scoring System-real time) score according to gender. This study aimed to address this precise question. Methods: The study included recreational athletes aged 18-25 years who did not have a history of musculoskeletal injury in the last 6 months or surgery or fracture in the last 24 months, and who were classified as active according to the General Practice Physical Activity Questionnaire. Participants were evaluated using the LESS-RT. Jump-landing errors were evaluated in real-time by a sports medicine specialist. Results: The study included 55 healthy individuals (28 females, 27 males) with an average age of 21.8 ± 1.1 years. The two sexes were not significantly different in terms of age (p=0.572). For all participants, the mean LESS-RT score was 6.93 ± 1.99. Females scored significantly higher than males (7.61 ± 1.81 vs. 6.22 ± 1.97, p=0.009). However, the LESS-RT scores were not significantly correlated with anthropometric characteristics, such as age, height, body weight, or BMI, according to sex (p>0.05). Females scored higher on LESS-RT (i.e. poorer performance), (p= 0.007). Conclusion: Female athletes scored higher on LESS-RT. Athletes, especially females, should be assessed for biomechanical errors in jump-landing.