Objective In this study linalool (LN), which has antihyperglycemic, hypolipidemic and antioxidant properties, is intended to be used in the treatment of neurodegenerations and neural disorders that may occur due to rifampicin (RF). For this reason, it was aimed to examine the effects of LN on the expression of genes, biochemical and histopathological parameters in these metabolic pathways against neurotoxicity that may occur due to RF, and to investigate the protective effects of LN, which has antioxidant properties. Material and Method Thirty healthy male Spraque-Dawley rats were divided into five groups (group 1; control, group 2; solvent control (DMSO); group 3, RF; group 4, LN; group 5; RF+LN). Brain tissues were taken for biochemical, histological and gene expressions analyses. Blood samples were taken to measure blood glucose levels. Results Rifampicin treatment significantly increased CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels, while reducing brain weight according to findings. On the other hand, there was a significant decrease in CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels in the RF+LN group, while a significant improvement in brain weight was observed and as a result of histological analyzes, it was observed that the damage caused by RF decreased in the groups given LN. Conclusion LN was found to be highly effective in protecting the brain from the toxic effects of RF.