The global Covid-19 pandemic had a devastating impact on our lives. The pandemic led to sudden and momentous changes in mobility styles and travel habits. Many users started preferring to travel via private vehicles, which is contrary to sustainability policies. Strict measures were implemented against the Covid-19 pandemic in Turkey during this process, as was the case all over the world. Taking into consideration these realities, the aim of the present study was to examine the impacts of the Covid-19 pandemic on the travel modes (public transportation, walking, and bicycle), anxiety and stress perceptions, and working conditions of individuals. A survey was conducted to measure the impacts of the pandemic and the measures taken. The sample of this study consists of people who regularly attended Suleyman Demirel University in Isparta and Akdeniz University in Antalya, Turkey, before the pandemic. An online survey was conducted for the case study during the May–June 2021 semester, and the survey was representative of the study population with a total of 556 participants. The travel time for both different university students decreased during the pandemic period. The percentage of those traveling to the university with their own cars increased to 77 % in AU and 66.8 % in SDU. The use of public transportation dropped to 6.1 % in AU and 11.8 % in SDU. 56.97 % of AU participants and 51.15 % of SDU participants reported that their walking habits decreased. It was reported that 52.73 % of the respondents in AU and 55.75 % of the respondents in SDU did not change their bicycle usage frequency. 64.24 % of AU and 74.42 % of SDU reported that their anxiety levels increased during the pandemic period. And for each of these analyses, there was no statistical difference between the two different university students.