<p><span style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;">Aim: Fluopyram is a mitochondrial complex II inhibitor with low water solubility and a relatively long half-life in soil. So it may also be dangerous for humans. The effect of fluopyram on DNA damage was evaluated in human lymphocytes using the comet assay.</span><br style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;"><span style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;">Materials and Methods: Lymphocytes of eight volunteers were isolated using histopaque-1077. Fluopyram was administered at doses of 0.05, 0.25, and 1.00 mg/mL for 1, 2, and 4 h. The comet assay was applied, and photographs of the slides were taken under a fluorescence microscope. 50 cells per slide were analyzed using the OpenComet software. The obtained results were statistically evaluated using one-way ANOVA.</span><br style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;"><span style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;">Results: Fluopyram treatments at 1.00 mg/mL for 1 h and 0.05, 0.25, and 1.00 mg/mL for 2 and 4 h resulted in a statistically significant increase in DNA damage compared to the internal control groups (p&lt;0.05). When comparing groups with the same treatment time but different doses, the increase in DNA damage observed after a 1-h treatment of 1.00 mg/mL fluopyram was higher than the increase observed after a 1-h treatment of 0.05 mg/mL fluopyram (p&lt;0.05). When comparing groups with different treatment times but the same dose, the increase in DNA damage after a 4-h treatment of 0.25 mg/mL fluopyram was higher than the increase observed after a 1-h treatment of 0.25 mg/mL fluopyram (p&lt;0.05).</span><br style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;"><span style="color: rgb(17, 17, 17); font-family: Poppins; font-size: 14px;">Conclusions: The results suggest that fluopyram causes an increase in DNA damage in a dose- and time-dependent manner. It is essential to investigate these findings in vivo as well.</span><br></p>