Objective: This study aimed to investigate whether gestationaldiabetes mellitus (GDM) affects the corneal biomechanical parameters. Material-Method: Thirty-eight pregnant women, newly diagnosed with GDMand, age- and gestational age-matched 34 healthy pregnant females were enrolledin the study. The right eye of the participants was examined. The cornealparameters including corneal hysteresis (CH), corneal resistance factor (CRF),Goldmann-correlated intraocular pressure (IOPg), and the corneal compensatedIOP (IOPcc), obtained by The Ocular Response Analyzer (ORA) device werecompared statistically.Results: The mean and gestational age of the participants was 30.71  ±5.09 years, 25.95  ±1.56 weeks and 29.97  ±4.10 years, 25.76  ±1.30 weeks in pregnant females with GDM andhealthy pregnant females, respectively. No significant differences in age(p=0.502) or gestational age (p=0.594) were noted. The values of CH, CRF, IOPg,and IOPcc were determined to be higher in pregnant females with GDM as comparedto that in healthy pregnant females. However, there was no statisticallysignificant difference in any other parameter (p=0.183, p=0.303, p=0.732 andp=0.330, respectively).Conclusions: Short-term hyperglycemia does not affect the cornealbiomechanical parameters.