The main objective of this study was to estimate the economic losses resulting from overdose used of pesticide and determining of economic costs on practices of pesticide application and level of pesticide use on oil rose production in Turkey. Lakes Region of Turkey was chosen as research area because oil rose is a major crop grown in the study area. The data used in this study were collected from oil rose growers using a face-to-face interview. The result of the analyses showed that farmers use agricultural chemical more than the recommendations of extension units, depending on the prospectus of chemicals in the oil rose production. The average usages per hectare of active ingredient of insecticides and fungicides were determined to be 2 612.80 and 2 347.50 gr-ml-cc, respectively. An average plant protection cost was a,not sign 593.31 ha(-1), which was 25.17 % of total oil rose production costs. It was calculated that economic loss was a,not sign 172.37 per hectare due to overdose of agricultural chemicals. The percentages of this loss were 58.66 %, 37.56 % and 3.78 % copper sulfate, pesticides and sulfur, respectively. It was calculated that economic loss was a,not sign 64.73 per hectare due to overdose of pesticides. The percentages of this loss are 61.40 % and 38.60 % insecticide and fungicide, respectively. Gain threshold was calculated as 637.75 kg ha(-1).